Gerade B B, Lee S H, Scott T W, Edman J D, Harrington L C, Kitthawee S, Jones J W, Clark J M
Department of Entomology, University of Massachusetts, Amherst, MA 01003, USA.
J Med Entomol. 2004 Mar;41(2):231-8. doi: 10.1603/0022-2585-41.2.231.
In previous studies, we developed linear regression models to age-grade female Aedes aegypti L. reared and maintained under controlled laboratory conditions. The models were based on temporal differences between two cuticular hydrocarbons, pentacosane (C25H52) and nonacosane (C29H60), which were extracted from Ae. aegypti legs and analyzed by gas-liquid chromatography. These initial models predicted adult female age up to 165 DD (12-15 calendar d at 28 degrees C). The age of older mosquitoes, however, could not be accurately predicted. In this study, our original regression models were tested using age data obtained from mosquitoes maintained in a field laboratory and those that were marked, released, and recaptured in northwestern Thailand. Our field data led to the development of two new regression models: one for the cool-dry season (February-March) and one for the rainy season (July-August). Both models resulted in better estimates of age than the original model and thus improved our ability to predict the age of Ae. aegypti to 15 calendar d. Females older than 15 d can be identified as such, but their exact age cannot yet be estimated. The new models will be useful for epidemiological studies and evaluating the impact of Ae. aegypti control interventions for disease prevention.
在先前的研究中，我们建立了线性回归模型，用于对在实验室控制条件下饲养和维持的雌性埃及伊蚊进行年龄分级。这些模型基于从埃及伊蚊腿部提取并通过气液色谱分析的两种表皮碳氢化合物，二十五烷（C25H52）和二十九烷（C29H60）之间的时间差异。这些初始模型可预测成年雌性埃及伊蚊的年龄，最长可达165度日（在28摄氏度下为12 - 15个日历日）。然而，年龄较大的蚊子的年龄无法准确预测。在本研究中，我们使用从野外实验室饲养的蚊子以及在泰国西北部标记、释放和重新捕获的蚊子获得的年龄数据，对我们原来的回归模型进行了测试。我们的野外数据促成了两个新的回归模型的开发：一个用于凉爽干燥季节（2月至3月），一个用于雨季（7月至8月）。这两个模型对年龄的估计都比原始模型更好，从而提高了我们预测埃及伊蚊年龄至15个日历日的能力。15日龄以上的雌性蚊子可以被识别出来，但它们的确切年龄尚无法估计。这些新模型将有助于流行病学研究以及评估埃及伊蚊控制干预措施对疾病预防的影响。
