Devleeschouwer Nicole, Libeer Jean Claude, Martens Frank K, Neels Hugo, Van Damme Marc, Verstraete Alain, Deveaux Marc, Wallemacq Pierre E
Institute of Public Health, Department of Clinical Biology, 14 Rue J. Wytsman, 1050 Brussels, Belgium.
Clin Chem Lab Med. 2004 Jan;42(1):57-61. doi: 10.1515/CCLM.2004.011.
This study describes the performance of the different methods used for blood alcohol analysis by the Belgian laboratories participating in the external quality assessment scheme. The best performance was achieved by the gas chromatography (GC) and the enzymatic methods. The enzymatic methods are easier to use but more apparatus-dependent, whereas GC methods display higher precision, especially at concentrations near the critical legal limit of 0.5 g/l. The study highlights the need to introduce a methodology other than the chemical oxidation-based method of Casier, the sole legal method for forensic analyses in drinking-driving cases in Belgium. This method with poor intra-laboratory, inter-laboratory and linearity performances has definitely become obsolete. Even though GC clearly appears as the method of choice, mainly due to its higher specificity, the present study also shows the need for practical guidelines to improve the quality of GC alcohol analyses used in Belgium.
本研究描述了参与外部质量评估计划的比利时实验室用于血液酒精分析的不同方法的性能。气相色谱法（GC）和酶法的性能最佳。酶法使用起来更简便，但对仪器的依赖性更强，而气相色谱法显示出更高的精密度，尤其是在接近0.5 g/l这一关键法定限值的浓度下。该研究强调，有必要引入一种不同于基于化学氧化的卡西耶方法的方法，卡西耶方法是比利时酒驾案件法医分析的唯一法定方法。这种方法在实验室内、实验室间和线性方面表现不佳，显然已经过时。尽管气相色谱法由于其更高的特异性明显是首选方法，但本研究也表明需要实用指南来提高比利时使用的气相色谱酒精分析的质量。
