来自安得拉邦本土天然基质的生物活性稀有放线菌。

Bioactive rare actinomycetes from indigenous natural substrates of Andhra Pradesh.

作者信息

Ellaiah P, Raju K V V S N Bapi, Adinarayana K, Adinarayana G, Prabhakar T, Premkumar J

机构信息

Pharmaceutical Biotechnology Division, Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530 003, India.

出版信息

Hindustan Antibiot Bull. 2002 Feb-Nov;44(1-4):17-24.

PMID:15061589

Abstract

This investigation reports the occurrence of bioactive rare actinomycetes from different indigenous natural substrates of Andhra Pradesh. During the course of our investigation on 20 terrestrial soil samples, 5 marine samples and 3 fresh water samples, a total of 92 rare actinomycetes belonging to Micromonospora, Nocardia, Actinomadura and Thermoactinomyces genera were isolated. The antimicrobial and enzymatic activities were studied for all the isolates. The antibacterial and antifungal activities were studied preliminarily by cross-streak method followed by submerged fermentation studies employing standard cup-plate method for the assay. It was found that 34 isolates (36.95%) showed excellent antibacterial activity and 29 isolates (31.52%) showed good antifungal activity. Proteolytic and amylolytic activities were also studied. It was observed that 79 isolates (85.86%) showed proteolytic activity and 75 isolates (81.52%) showed amylolytic activity.

摘要

本调查报道了从安得拉邦不同本土天然基质中分离出具有生物活性的稀有放线菌。在对20份陆地土壤样品、5份海洋样品和3份淡水样品的调查过程中，共分离出92株属于小单孢菌属、诺卡氏菌属、马杜拉放线菌属和嗜热放线菌属的稀有放线菌。对所有分离株进行了抗菌和酶活性研究。通过交叉划线法初步研究了抗菌和抗真菌活性，随后采用标准杯碟法进行液体发酵研究以进行测定。结果发现，34株分离株（36.95%）表现出优异的抗菌活性，29株分离株（31.52%）表现出良好的抗真菌活性。还研究了蛋白水解和淀粉水解活性。观察到79株分离株（85.86%）表现出蛋白水解活性，75株分离株（81.52%）表现出淀粉水解活性。