Tanimoto K, Ohno S, Imada M, Honda K, Ohno-Nakahara M, Kapila S, Tanne K
Department of Orthodontics & Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, 734-8553 Hiroshima, Japan.
J Oral Pathol Med. 2004 Apr;33(4):218-23. doi: 10.1111/j.0904-2512.2004.00097.x.
Pyridinoline (Pyr) and deoxypyridinoline (Dpyr) collagen cross-links are known markers of bone and cartilage turnover that are found in urine in various diseases. The present study was designed to quantify Pyr and Dpyr levels in urine of patients with osteoarthritis (OA) of the temporomandibular joint (TMJ), and to evaluate whether their concentrations are related to specific pathologic findings in the TMJ.
Urine samples were obtained from 12 patients with OA of the TMJ and 16 asymptomatic controls, and following appropriate preparation, analyzed by high-performance liquid chromatography (HPLC) and fluorescence spectroscopy for Pyr and Dpyr.
The urinary concentration of Pyr and the Pyr to Dpyr (Pyr/Dpyr) ratio were significantly higher (P < 0.05) in OA patients than in the controls (182.2 +/- 86.5 pmol/ml vs. 115.6 +/- 27.9 pmol/ml and 4.00 +/- 1.53 pmol/ml vs. 2.86 +/- 0.97 pmol/ml, respectively). However, the Pyr/Dpyr ratio was not associated with any specific clinical or radiographic findings.
These findings suggest that the level of Pyr and the Pyr/Dpyr ratio in urine may be a useful diagnostic indicator for intra-articular pathologic changes during TMJ OA.
吡啶啉（Pyr）和脱氧吡啶啉（Dpyr）是已知的骨和软骨转换标志物，在多种疾病的尿液中均可检测到。本研究旨在定量颞下颌关节（TMJ）骨关节炎（OA）患者尿液中的Pyr和Dpyr水平，并评估其浓度是否与TMJ的特定病理表现相关。
收集12例TMJ OA患者和16例无症状对照者的尿液样本，经过适当处理后，采用高效液相色谱（HPLC）和荧光光谱法分析Pyr和Dpyr。
OA患者尿液中Pyr浓度及Pyr与Dpyr的比值（Pyr/Dpyr）均显著高于对照组（分别为182.2±86.5 pmol/ml对115.6±27.9 pmol/ml以及4.00±1.53 pmol/ml对2.86±0.97 pmol/ml，P<0.05）。然而，Pyr/Dpyr比值与任何特定的临床或影像学表现均无关联。
这些发现表明，尿液中Pyr水平及Pyr/Dpyr比值可能是TMJ OA期间关节内病理变化的有用诊断指标。
