Söderlund Johan, Nilsson Charlotta, Loré Karin, Castanos-Velez Esmeralda, Ekman Marianne, Heiden Thomas, Biberfeld Gunnel, Andersson Jan, Biberfeld Peter
Cancer Center Karolinska, Karolinska Hospital, Stockholm, Sweden.
J Med Primatol. 2004 Feb;33(1):16-24. doi: 10.1111/j.1600-0684.2003.00053.x.
The prevalence and differentiation of dendritic cells (DC) in lymphoid tissue of simian immunodeficiency virus (SIV)-infected cynomolgus monkeys was studied during disease progression. Lymph node biopsies were consecutively obtained from clinical rapid and slow progressors until the development of disease consistent with simian acquired immunodeficiency syndrome (sAIDS) occurred. Quantitative evaluation of CD1a+ DC and the expression of DC antigens related to maturation (CD83, DC-LAMP and S100b) were performed at the single cell level by in situ image analysis. Despite a persistent prevalence of CD1a+ DC in lymphoid tissue during disease progression, there was a subsequent drop of mature CD83+, DC-LAMP+ and S100b+ DC, correlating with the decline of CD4+ T cells in blood. Thus, disease progression to sAIDS was associated with impaired maturation of DC, and lack of CD83, DC-LAMP and S100b expression.
在疾病进展过程中，研究了感染猿猴免疫缺陷病毒（SIV）的食蟹猴淋巴组织中树突状细胞（DC）的患病率和分化情况。从临床快速进展者和缓慢进展者连续获取淋巴结活检样本，直至出现与猿猴获得性免疫缺陷综合征（sAIDS）相符的疾病。通过原位图像分析在单细胞水平对CD1a + DC进行定量评估，并检测与成熟相关的DC抗原（CD83、DC-LAMP和S100b）的表达。尽管在疾病进展过程中淋巴组织中CD1a + DC持续存在，但随后成熟的CD83 +、DC-LAMP +和S100b + DC数量下降，这与血液中CD4 + T细胞的减少相关。因此，疾病进展为sAIDS与DC成熟受损以及缺乏CD83、DC-LAMP和S100b表达有关。
