Vandewoestijne S, Baguette M, Brakefield P M, Saccheri I J
Centre for Biodiversity Research, Catholic University Louvain, Place Croix du Sud 5, B-1348 Louvain-la-Neuve, Belgium.
Mol Phylogenet Evol. 2004 May;31(2):630-46. doi: 10.1016/j.ympev.2003.09.007.
Mitochondrial DNA (mtDNA) sequences of the COI gene and the control region were used to examine the genetic population structure of Aglais urticae L. (Lepidoptera) over its entire geographic range, i.e., the Palaearctic. The phylogenetic relationships within and between A. urticae subspecies were determined and patterns of mtDNA divergence and ecological differentiation were compared. High gene flow together with a recent and sudden population expansion characterise the genetic population structure of this species. No geographically induced differentiation was observed, nor were subspecies identified as separate evolutionary units. The discrepancy between the genetic and ecological variation is most likely due to the slower rate of mtDNA evolution compared to ecological differentiation. The control region proved to be a less useful molecular marker for the population genetics and the phylogenetic reconstruction of closely related taxa in A. urticae than it has for other species. The extreme bias in adenine and thymine content (A+T=90.91%) probably renders this region highly susceptible to homoplasy, resulting in a less informative molecular marker.
利用细胞色素氧化酶亚基I（COI）基因和控制区的线粒体DNA（mtDNA）序列，研究荨麻蛱蝶（鳞翅目）在其整个地理分布范围，即古北区的遗传种群结构。确定了荨麻蛱蝶亚种内部和之间的系统发育关系，并比较了mtDNA分歧模式和生态分化模式。高基因流以及近期突然的种群扩张是该物种遗传种群结构的特征。未观察到地理诱导分化，也未将亚种鉴定为独立的进化单元。遗传变异与生态变异之间的差异很可能是由于mtDNA进化速度比生态分化速度慢。与其他物种相比，控制区被证明是荨麻蛱蝶种群遗传学和近缘类群系统发育重建中不太有用的分子标记。腺嘌呤和胸腺嘧啶含量的极端偏差（A+T=90.91%）可能使该区域极易发生平行进化，导致分子标记信息较少。
