Meyran J C, Monnerot M, Taberlet P
Laboratoire de Biologie des Populations d'altitude, URA UMR 5553, Université J. Fourier, Grenoble, France.
Mol Phylogenet Evol. 1997 Aug;8(1):1-10. doi: 10.1006/mpev.1996.0399.
Mitochondrial DNA nucleotide sequence analysis was used to determine the extent of genetic differentiation among six species of the genus Gammarus (Crustacea, Amphipoda) common in France (G. fossarum, G. pulex, G. lacustris, G. wautieri, G. roeseli, G. marinus). From 23 different populations, 104 specimens were compared to examine their taxonomic status and their phylogenetic relationships. Nucleotide sequences for a 376-base segment of the cytochrome c oxidase subunit I (COI) gene were obtained through direct sequencing of amplified DNA. The strong sequence variability found at the interspecific level, generally masked by morphological or ecological similarities, is consistent with that observed for other crustaceans and supports existing species classifications. A high level of genetic variation was observed at the intraspecific level, suggesting that the COI gene will be a powerful marker for amphipod population biology. Based on the mitochondrial population biology. Based on the mitochondrial DNA results, we propose a phylogenetic hypothesis of the relationships among the six studied species, which is discussed in biogeographic and evolutionary terms.
利用线粒体DNA核苷酸序列分析来确定法国常见的六种钩虾属（甲壳纲，端足目）物种（深水钩虾、蚤状钩虾、湖钩虾、沃氏钩虾、罗氏钩虾、海钩虾）之间的遗传分化程度。从23个不同种群中选取了104个标本进行比较，以研究它们的分类地位及其系统发育关系。通过对扩增DNA进行直接测序，获得了细胞色素c氧化酶亚基I（COI）基因376个碱基片段的核苷酸序列。在种间水平发现的强烈序列变异性，通常被形态学或生态学上的相似性所掩盖，这与其他甲壳类动物的情况一致，并支持现有的物种分类。在种内水平观察到高度的遗传变异，这表明COI基因将成为端足目种群生物学的一个有力标记。基于线粒体种群生物学。基于线粒体DNA结果，我们提出了所研究的六个物种之间关系的系统发育假说，并从生物地理学和进化角度进行了讨论。
