Giannoutsou E P, Meintanis C, Karagouni A D
Department of Botany, Faculty of Biology, University of Athens, 15781 Athens, Greece.
Bioresour Technol. 2004 Jul;93(3):301-6. doi: 10.1016/j.biortech.2003.10.023.
A dynamic fed-batch microcosm system is described which permits assessment of the progressive growth of yeasts through olive oil waste. We report on its application to measure the effects of the growth of yeast strains upon the chemical composition of "alpeorujo", the waste of a two-phase decanter system used for the extraction of olive oil. Six phenotypically distinct groups of yeasts were isolated. Three selected isolates were identified as being most closely related to Saccharomyces sp., Candida boidinii and Geotrichum candidum using biochemical tests and partial 18S rDNA gene sequence analysis. This is the first report of yeast growth on "alpeorujo" by the use of a fed-batch microcosm system, resulting in the change of the initial chemical composition of "alpeorujo" and in the decrease of the toxic substances such as phenols.
描述了一种动态补料分批微观系统，该系统允许评估酵母在橄榄油废料中的逐步生长情况。我们报告了其在测量酵母菌株生长对“alpeorujo”（用于提取橄榄油的两相倾析系统的废料）化学成分影响方面的应用。分离出了六个表型不同的酵母组。使用生化测试和部分18S rDNA基因序列分析，确定三个选定的分离株与酿酒酵母属、博伊丁假丝酵母和白地霉关系最为密切。这是首次关于使用补料分批微观系统研究酵母在“alpeorujo”上生长的报告，该研究导致“alpeorujo”初始化学成分发生变化，酚类等有毒物质减少。
