Fryssouli Vassiliki, Kefalogianni Io, Polemis Elias, Typas Milton A, Zervakis Georgios I
Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
Section of Genetics and Biotechnology, Department of Biology, National and Kapodistrian University of Athens, Panepistemiopolis, 15701 Athens, Greece.
J Fungi (Basel). 2025 Sep 22;11(9):687. doi: 10.3390/jof11090687.
This study investigates the diversity and provides a preliminary evaluation of the enzymatic potential of culturable fungi present in two-phase olive mill waste (TPOMW), a lignocellulose- and phenolic-rich agro-industrial by-product generated in large quantities in olive oil-producing countries. Ninety-four isolates, representing 31 species of the phyla Ascomycota, Basidiomycota, and Mucoromycota, were obtained and identified by using ITS, 28S, 1-α, 2, 2, , and/or sequences. Among the identified taxa, two new species within the Harzianum clade, namely (phylogenetically related to and ) and (phylogenetically related to ), were described following a multilocus phylogenetic analysis combined with a study of their morphoanatomical features. A rather high phylogenetic divergence was detected in , and , while , , and were recorded for the first time in TPOMW. A preliminary screening revealed 29 species with cellulolytic and/or xylanolytic activities; 26 species displayed dye decolorization capacity, while ligninolytic and laccase activities were restricted to a few taxa. The most promising degraders of lignocellulosics included strains of , , , and . Fungi detected in TPOMW are promising biotechnological tools to be exploited in the frame of circular economy applications.
本研究调查了两相橄榄果渣（TPOMW）中可培养真菌的多样性，并对其酶潜力进行了初步评估。TPOMW是一种富含木质纤维素和酚类物质的农业工业副产品，在橄榄油生产国大量产生。通过使用ITS、28S、1-α、2、2、 及/或 序列，获得并鉴定了94株分离物，它们代表了子囊菌门、担子菌门和毛霉门的31个物种。在已鉴定的分类群中，通过多位点系统发育分析并结合其形态解剖特征研究，描述了哈茨木霉属分支内的两个新物种，即 （在系统发育上与 和 相关）和 （在系统发育上与 相关）。在 、 和 中检测到相当高的系统发育差异，而 、 、 和 是首次在TPOMW中记录到。初步筛选发现29个具有纤维素分解和/或木聚糖分解活性的物种；26个物种具有染料脱色能力，而木质素分解和漆酶活性仅限于少数分类群。最有前景的木质纤维素降解菌包括 、 、 和 的菌株。在TPOMW中检测到的真菌是在循环经济应用框架中有待开发的有前景的生物技术工具。
