Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, 60131, Ancona, Italy.
Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131, Ancona, Italy.
Sci Rep. 2019 Nov 18;9(1):16966. doi: 10.1038/s41598-019-53313-z.
The effect of inoculated azotobacteria and basidiomycetes white-rot fungi on the population dynamics of bacteria and eumycetes during the co-composting of olive mill pomace and wheat straw was evaluated by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) analysis combined with sequencing of rRNA gene amplicons from selected DGGE bands. The evolution of pH, temperature, phytotoxicity and water-soluble phenol content during co-composting was also monitored. In general, a similar evolution of microbial biodiversity was seen in both the inoculated and uninoculated (control) piles, which was in keeping with a similar evolution of phytotoxicity and water-soluble phenol content. Overall, under the conditions applied, data suggest a marginal influence of the inoculated starters on the physical, chemical and microbiological properties of compost piles, with the resident microbiota playing a major role.
通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析结合从选定的 DGGE 条带的 rRNA 基因扩增子测序，评估了接种固氮菌和担子菌白腐真菌对橄榄渣和麦秸共堆肥过程中细菌和真菌种群动态的影响。还监测了共堆肥过程中 pH 值、温度、植物毒性和水溶性苯酚含量的演变。一般来说，接种和未接种（对照）堆肥中的微生物生物多样性的演变相似，这与植物毒性和水溶性苯酚含量的演变相似。总的来说，在所应用的条件下，数据表明接种的启动子对堆肥物理、化学和微生物特性的影响很小，而驻留的微生物群起着主要作用。
