Njeru C A, McDowell L R, Wilkinson N S, Linda S B, Williams S N, Lentz E L
Department of Animal Science, University of Florida, Gainesville 32611.
J Anim Sci. 1992 Aug;70(8):2562-7. doi: 10.2527/1992.7082562x.
Forty-three crossbred wethers weighing 35 to 60 kg were used to investigate the effect of a single i.m. injection of DL-alpha-tocopherol (DL-alpha-ol). Animals were offered 1 kg/d of a basal diet containing 25 ppm of vitamin E. Lambs were randomly assigned to one of five DL-alpha-ol injection treatments as follows: 1) control (placebo, 0 IU), 2) 125 IU, 3) 250 IU, 4) 500 IU, or 5) 1,000 IU. Blood samples were taken via jugular venipuncture on d 1 before treatment administration and thereafter at designated intervals up to 360 h postinjection. The i.m. injections of DL-alpha-ol irrespective of dose increased serum alpha-tocopherol. Results showed a dose x time interaction (P less than .0001) across all treatments. Serum alpha-tocopherol increased rapidly to maximum concentration during the first 8 to 12 h for all non-zero treatments, followed by a rapid decline to pretreatment values. The mean serum alpha-tocopherol concentration at 0 h was .69 microgram/mL. Estimated peak serum alpha-tocopherol concentrations +/- SE were 6.68 +/- 1.04, 9.62 +/- 1.04, 21.66 +/- 2.37, and 50.75 +/- 7.05 micrograms/mL for Treatments 2 through 5, respectively. Results showed a quadratic dose effect (P less than .0003) on maximum response with apparently no effect on time taken to reach this peak. There was also a quadratic dose effect (P less than .0001) on the area under the concentration-time curve. The time taken for serum alpha-tocopherol to return to pretreatment levels increased with dose (56, 64, 67, and 74 h, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
选用43只体重在35至60千克之间的杂交阉羊，研究肌肉注射一次DL-α-生育酚（DL-α-ol）的效果。给动物提供每日1千克含25 ppm维生素E的基础日粮。羔羊被随机分为以下五种DL-α-ol注射处理组之一：1）对照组（安慰剂，0 IU），2）125 IU，3）250 IU，4）500 IU，或5）1000 IU。在给药前第1天通过颈静脉穿刺采集血样，此后在注射后长达360小时的指定时间间隔采集血样。无论剂量如何，肌肉注射DL-α-ol均可提高血清α-生育酚水平。结果显示，所有处理组均存在剂量×时间交互作用（P＜0.0001）。对于所有非零处理组，血清α-生育酚在最初8至12小时内迅速升高至最高浓度，随后迅速降至预处理值。0小时时血清α-生育酚的平均浓度为0.69微克/毫升。处理2至5的血清α-生育酚估计峰值浓度±标准误分别为6.68±1.04、9.62±1.04、21.66±2.37和50.75±7.05微克/毫升。结果显示，最大反应存在二次剂量效应（P＜0.0003），而达到该峰值的时间显然不受影响。浓度-时间曲线下面积也存在二次剂量效应（P＜0.0001）。血清α-生育酚恢复至预处理水平所需的时间随剂量增加而增加（分别为56、64、67和74小时）。（摘要截断于250字）
