Ochoa L, McDowell L R, Williams S N, Wilkinson N, Boucher J, Lentz E L
Department of Animal Science, University of Florida, Gainesville 32611.
J Anim Sci. 1992 Aug;70(8):2568-73. doi: 10.2527/1992.7082568x.
Thirty-five crossbred wethers were used to determine the concentrations of alpha-tocopherol in serum and tissues after oral supplementation of six different vitamin E product forms. Five wethers were assigned to each of the following treatments: 1) control, no supplemental vitamin E (C), 2) emulsifiable DL-alpha-tocopheryl acetate-dry (Rovimix E-50% SD), 3) nonemulsifiable DL-alpha-tocopheryl acetate-dry (Rovimix E-50% Ads), 4) emulsifiable DL-alpha-tocopheryl acetate-liquid (Rovimix E-40% Dispersible Liquid Concentrate [DLC]); 5) emulsifiable DL-alpha-tocopherol-liquid (Hoffmann-La Roche, E-40% DLC alcohol), 6) micellized DL-alpha-tocopheryl acetate-liquid (Bioglan, Inc., E-20%); and 7) micellized DL-alpha-tocopherol-liquid (Bioglan, Inc., E-20%). Animals were supplemented daily with 1,000 IU of their respective vitamin E sources for 56 d. Blood samples were collected daily from d 0 to 7 and weekly until d 56. Animals were subsequently killed by exsanguination after stunning and eight different tissues were collected for alpha-tocopherol analysis. There were effects of day, treatment, and day x treatment interaction on serum alpha-tocopherol. All supplemented groups were higher in serum alpha-tocopherol concentration than were the C wethers. The emulsifiable vitamin E alcohol liquid product form (Treatment 5) yielded higher (P less than .01) serum alpha-tocopherol concentration than the emulsifiable acetate liquid product (Treatment 4). Sheep on Treatment 5 reached maximum concentration on d 1, sheep on Treatment 6 on d 2, and the sheep on the remaining Treatments by d 3. Blood sera alpha-tocopherol concentrations stabilized by d 6 in all supplemented groups.(ABSTRACT TRUNCATED AT 250 WORDS)
选用35只杂交阉羊，以确定口服六种不同形式的维生素E产品后血清和组织中α-生育酚的浓度。将五只阉羊分配到以下每种处理组：1）对照组，不补充维生素E（C）；2）可乳化的DL-α-生育酚醋酸酯干粉（罗氏维生素E-50% SD）；3）不可乳化的DL-α-生育酚醋酸酯干粉（罗氏维生素E-50% Ads）；4）可乳化的DL-α-生育酚醋酸酯液体（罗氏维生素E-40% 分散液浓缩物[DLC]）；5）可乳化的DL-生育酚液体（霍夫曼-罗氏公司，E-40% DLC酒精溶液）；6）胶束化的DL-α-生育酚醋酸酯液体（Bioglan公司，E-20%）；7）胶束化的DL-生育酚液体（Bioglan公司，E-20%）。动物每天补充1000 IU各自的维生素E来源，持续56天。从第0天到第7天每天采集血样，之后每周采集一次，直至第56天。随后，动物在致昏后放血处死，收集八个不同组织进行α-生育酚分析。日、处理组以及日×处理组交互作用对血清α-生育酚有影响。所有补充组的血清α-生育酚浓度均高于C组阉羊。可乳化的维生素E酒精液体产品形式（处理组5）产生的血清α-生育酚浓度高于可乳化的醋酸酯液体产品（处理组4）（P<0.01）。处理组5的绵羊在第1天达到最高浓度，处理组6的绵羊在第2天达到最高浓度，其余处理组的绵羊在第3天达到最高浓度。所有补充组的血清α-生育酚浓度在第6天稳定下来。（摘要截短至250字）
