Langhans Bettina, Braunschweiger Ingrid, Schweitzer Susann, Sauerbruch Tilman, Spengler Ulrich
Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany.
Vaccine. 2004 Mar 29;22(11-12):1441-7. doi: 10.1016/j.vaccine.2003.10.014.
We analysed whether hepatitis C virus (HCV)-specific antibody producing B lymphocytes can be induced in vitro with HCV-derived lipopeptides containing different T helper cell epitopes. HCV-specific antibody producing B cells were detected by ELISPOT at the single cell level. HCV-derived lipopeptides, but not their corresponding unlipidated peptides, induced B lymphocytes, which produced antibodies mainly reacting with the HCV-derived lipopeptides. The number of antigen-specific B cells was dependent on the number of added autologous T helper lymphocytes during the incubation period. Thus, HCV lipopeptides are more immunogenic than unmodified peptides and can induce HCV-reactive B lymphocytes in antigen-naïve lymphocytes.
我们分析了是否可以在体外使用含有不同T辅助细胞表位的丙型肝炎病毒（HCV）衍生脂肽诱导产生HCV特异性抗体的B淋巴细胞。通过ELISPOT在单细胞水平检测产生HCV特异性抗体的B细胞。HCV衍生脂肽而非其相应的未脂质化肽可诱导B淋巴细胞，这些B淋巴细胞产生的抗体主要与HCV衍生脂肽发生反应。抗原特异性B细胞的数量取决于孵育期添加的自体T辅助淋巴细胞的数量。因此，HCV脂肽比未修饰的肽具有更强的免疫原性，并且可以在未接触过抗原的淋巴细胞中诱导产生HCV反应性B淋巴细胞。
