Rees Helen C, Grant William D, Jones Brian E, Heaphy Shaun
Department of Microbiology and Immunology, University of Leicester, University Road, LE1 9HN, Leicester, UK.
Extremophiles. 2004 Feb;8(1):63-71. doi: 10.1007/s00792-003-0361-4. Epub 2003 Oct 29.
DNA was extracted from water and sediment samples taken from soda lakes of the Kenyan-Tanzanian Rift Valley. DNA was also extracted from microbial enrichment cultures of sediment samples. 16S rRNA genes were amplified by the polymerase chain reaction and microbial diversity was studied using denaturing gradient gel electrophoresis (DGGE) of 16S rDNA amplicons. Cloning and sequencing of single DGGE bands showed that they usually contained mixed amplicons. Several of the amplicon sequences had high identities, up to 99%, with 16S rRNA genes of organisms previously isolated from soda lakes, while others were only distantly related, with identities as low as 82%. Phylogenetic analysis of the sequenced amplicons indicated that sequences were related to the haloarchaeal, Bacillus/Clostridium, Rhodobacterium/ Thioalcalovibrio/ Methylobacter, and Cytophaga/Flavobacterium/Bacteroides (CFB) groups and the enterobacteria/ Aeromonas/Vibrio part of the gamma3 subdivision of the Proteobacteria.
从肯尼亚 - 坦桑尼亚裂谷带苏打湖采集的水和沉积物样本中提取DNA。也从沉积物样本的微生物富集培养物中提取DNA。通过聚合酶链反应扩增16S rRNA基因，并使用16S rDNA扩增子的变性梯度凝胶电泳（DGGE）研究微生物多样性。单个DGGE条带的克隆和测序表明，它们通常包含混合扩增子。一些扩增子序列与先前从苏打湖分离出的生物体的16S rRNA基因具有高达99%的高度同一性，而其他序列则亲缘关系较远，同一性低至82%。对测序扩增子的系统发育分析表明，这些序列与嗜盐古菌、芽孢杆菌/梭菌、红杆菌/硫碱弧菌/甲基杆菌以及噬纤维菌/黄杆菌/拟杆菌（CFB）组以及变形菌门γ3亚群的肠杆菌/气单胞菌/弧菌部分相关。
