Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Department of Zoology, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
PLoS One. 2019 Apr 25;14(4):e0215734. doi: 10.1371/journal.pone.0215734. eCollection 2019.
As a step towards better understanding of diversity and biology of phages and their hosts in haloalkaline Lake Elmenteita, phages were isolated from sediment samples and overlying water using indigenous bacteria as hosts. 17 seemingly different phages of diverse morphotypes with different dimensions and partly exhibiting remarkably unusual ultrastructures were revealed by transmission electron microscopy. 12 clonal phage isolates were further characterized. Infection capability of the phages was optimum at 30-35°C and in alkali condition with optimum at pH 10-12. Structural protein profiles and restriction fragment length polymorphism analyses patterns were distinct for each of the phage type. Complete nucleotide sequences of phages vB-VmeM-32, vB_EauS-123 and vB_BhaS-171 genomes varied in size from 30,926-199,912 bp and G + C content of between 36.25-47.73%. A range of 56-260 potential open reading frames were identified and annotated. The results showed that the 12 phages were distinct from each other and confirmed the presence and diversity of phages in extreme environment of haloalkaline Lake Elmenteita. The phages were deposited at the German Collection of Microorganisms and Cell Cultures and three of their genomes uploaded to NCBI GenBank.
为了更好地了解 haloalkaline Lake Elmenteita 中噬菌体及其宿主的多样性和生物学特性，本研究采用土著细菌作为宿主，从沉积物样品和上覆水中分离噬菌体。透射电子显微镜显示，分离到了 17 种形态不同、尺寸不同、部分具有显著异常超微结构的似乎不同的噬菌体。进一步对 12 个克隆噬菌体分离物进行了特征描述。噬菌体的感染能力在 30-35°C 和碱性条件下最佳，最佳 pH 值为 10-12。每种噬菌体类型的结构蛋白图谱和限制性片段长度多态性分析模式都不同。噬菌体 vB-VmeM-32、vB_EauS-123 和 vB_BhaS-171 的基因组全长分别为 30926-199912bp，G+C 含量为 36.25-47.73%。鉴定和注释了 56-260 个潜在的开放阅读框。结果表明，这 12 种噬菌体彼此不同，证实了 haloalkaline Lake Elmenteita 极端环境中噬菌体的存在和多样性。这些噬菌体已被保藏在德国微生物和细胞培养物收藏中心，其中 3 个基因组已上传到 NCBI GenBank。
