Sjöling Sara, Cowan Don A
Department of Biochemistry and Molecular Biology, University College London, Gower St, London, WC1E 6BT, UK.
Extremophiles. 2003 Aug;7(4):275-82. doi: 10.1007/s00792-003-0321-z. Epub 2003 Apr 9.
The microbial diversity in maritime meltwater pond sediments from Bratina Island, Ross Sea, Antarctica was investigated by 16S rDNA-dependent molecular phylogeny. Investigations of the vertical distribution, phylogenetic composition, and spatial variability of Bacteria and Archaea in the sediment were carried out. Results revealed the presence of a highly diverse bacterial population and a significantly depth-related composition. Assessment of 173 partial 16S rDNA clones analyzed by amplified rDNA restriction analysis (ARDRA) using tetrameric restriction enzymes (HinP1I 5'G/CGC3'and Msp I. 5'C/CGG3', BioLabs) revealed 153 different bacterial OTUs (operational taxonomic units). However, only seven archaeal OTUs were detected, indicating low archaeal diversity. Based on ARDRA results, 30 bacterial clones were selected for sequencing and the sequenced clones fell into seven major lineages of the domain Bacteria; the alpha, gamma, and delta subdivisions of Proteobacteria, the Cytophaga-Flavobacterium-Bacteroides, the Spirochaetaceae, and the Actinobacteria. All of the archaeal clones sequenced belonged to the group Crenarchaeota and phylogenetic analysis revealed close relationships with members of the deep-branching Group 1 Marine Archaea.
通过基于16S rDNA的分子系统发育方法，对南极洲罗斯海布拉蒂纳岛海洋融水池塘沉积物中的微生物多样性进行了研究。对沉积物中细菌和古菌的垂直分布、系统发育组成及空间变异性进行了调查。结果显示存在高度多样的细菌群落，且其组成与深度显著相关。使用四聚体限制性内切酶（HinP1I 5'G/CGC3'和Msp I. 5'C/CGG3'，BioLabs）通过扩增rDNA限制性分析（ARDRA）对173个部分16S rDNA克隆进行分析，结果显示有153个不同的细菌操作分类单元（OTU）。然而，仅检测到7个古菌OTU，表明古菌多样性较低。基于ARDRA结果，选择30个细菌克隆进行测序，测序克隆分属于细菌域的7个主要谱系；变形菌门的α、γ和δ亚类、噬纤维菌-黄杆菌-拟杆菌类、螺旋体科和放线菌。所有测序的古菌克隆均属于泉古菌门，系统发育分析显示它们与深海分支的第1组海洋古菌成员关系密切。
