Siniavskaia M G, Danilenko N G, Davydenko O G, Ermishina N M, Bel'ko N B, Gordeĭ I A
Institute of Genetics and Cytology, Belarussian Academy of Sciences, Minsk, 220072 Belarus.
Genetika. 2004 Feb;40(2):218-23.
The mode of inheritance of chloroplast and mitochondrial DNA (mtDNA) in rye x triticale intergeneric hybrids has been studied with the use of specific PCR markers for loci 18S/5S and 3'rbcL in organelle DNA. In rye x triticale BC1, mtDNA copies of two types, paternal and maternal, have been found; in BC2 plants, only paternal mtDNA and chloroplast DNA (cpDNA) have been detected. Mechanisms determining the inheritance and/or differential amplification of organelles of a specific type are discussed.
利用针对细胞器DNA中18S/5S和3'rbcL位点的特异性PCR标记,研究了黑麦与小黑麦属间杂种中叶绿体和线粒体DNA(mtDNA)的遗传模式。在黑麦×小黑麦BC1代中,发现了两种类型的mtDNA拷贝,即父本型和母本型;在BC2代植株中,仅检测到父本mtDNA和叶绿体DNA(cpDNA)。本文讨论了决定特定类型细胞器遗传和/或差异扩增的机制。