Shimoni E, Ravid U, Shoham Y
Department of Food Engineering and Biotechnology, Technion-Israel Institute of Technology, Haifa, Israel.
J Biotechnol. 2000 Feb 28;78(1):1-9. doi: 10.1016/s0168-1656(99)00199-6.
Natural aroma compounds are of major interest to the flavor and fragrance industry. Due to the limited sources for natural aromas, there is a growing interest in developing alternative sources for natural aroma compounds, and in particular aromatic aldehydes. In several microbial species aromatic aldehydes are detected as intermediates in the degradation pathway of phenylpropanoids. Thus, bioconversion of phenylpropanoids is one possible route for the production of these aroma compounds. The present work describes the isolation of microbial strains, capable of producing vanillin from isoeugenol. Bacterial strains isolated from soil, were screened for their ability to transform isoeugenol to vanillin. One of these strains, strain B2, was found to produce high amounts of vanillin when grown in the presence of isoeugenol, and was also capable of growing on isoeugenol as the sole carbon source. Based on its fatty acids profile, strain B2 was identified as a Bacillus subtilis sp. The bioconversion capabilities of strain B2 were tested in growing cultures and cell free extracts. In the presence of isoeugenol, a growing cultures of B. subtilis B2 produced 0.61 g l-1 vanillin (molar yield of 12.4%), whereas cell free extracts resulted in 0.9 g l-1 vanillin (molar yield of 14%).
天然香气化合物是香料和香精行业的主要关注点。由于天然香料的来源有限，人们对开发天然香气化合物的替代来源，尤其是芳香醛，的兴趣日益浓厚。在几种微生物中，芳香醛被检测为苯丙烷类化合物降解途径中的中间体。因此，苯丙烷类化合物的生物转化是生产这些香气化合物的一种可能途径。本研究描述了能够从异丁香酚生产香草醛的微生物菌株的分离。从土壤中分离出的细菌菌株，被筛选其将异丁香酚转化为香草醛的能力。其中一株菌株B2，在异丁香酚存在下生长时被发现能产生大量香草醛，并且还能够以异丁香酚作为唯一碳源生长。根据其脂肪酸谱，菌株B2被鉴定为枯草芽孢杆菌。在生长培养物和无细胞提取物中测试了菌株B2的生物转化能力。在异丁香酚存在下，枯草芽孢杆菌B2的生长培养物产生了0.61 g l-1香草醛（摩尔产率为12.4%），而无细胞提取物产生了0.9 g l-1香草醛（摩尔产率为14%）。
