Iioka H, Hisanaga H, Akada S, Moriyama I, Ichijo M
Department of Obstetrics and Gynecology, Nara Medical University.
Nihon Sanka Fujinka Gakkai Zasshi. 1992 Jun;44(6):631-7.
We studied the platelet aggregation inhibiting activity and ADP degrading activity of human placental villi (tissue culture supernatant) and brush border membrane vesicles (BBMV) and obtained the following results. 1. There existed a platelet aggregation inhibiting activity in tissue culture supernatant of villi (S-villi) but not in that of decidua or amnion. The S-villi inhibited the platelet aggregation induced by ADP, but not that induced by collagen, arachidonic acid or ristocetin. And, there was also ADP degrading activity (ADPase activity) in the S-villi. ADP was quickly degraded by S-villi. When ADP was preincubated with S-villi, the platelet aggregation induced by ADP was completely lost. 2. There was very strong platelet aggregation inhibiting activity in placental BBMV. The BBMV almost completely inhibited the platelet aggregation induced by ADP, collagen, arachidonic acid and ristocetin. And there was very strong ADP degrading activity in the placental BBMV. ADP was quickly degraded by BBMV. When ADP was preincubated with BBMV, the platelet aggregation induced by ADP was completely lost. 3. The enzymatic character (heat stability, enzymatic kinetics, Ca++ dependency and pH dependency) of ADP degrading activity in BBMV was very similar to that in S-villi. 4. The ADP degrading activity of both S-villi and solubilized BBMV were fractionated by anion exchange column chromatography and gel filtration column chromatography in similar patterns, and it was shown that ADP degrading substance of both S-villi and solubilized BBMV had a molecular weight of about 60K.
我们研究了人胎盘绒毛（组织培养上清液）和刷状缘膜囊泡（BBMV）的血小板聚集抑制活性及ADP降解活性，结果如下。1. 绒毛组织培养上清液（S-绒毛）存在血小板聚集抑制活性，而蜕膜或羊膜的组织培养上清液则没有。S-绒毛可抑制ADP诱导的血小板聚集，但不能抑制胶原、花生四烯酸或瑞斯托霉素诱导的血小板聚集。此外，S-绒毛中还存在ADP降解活性（ADP酶活性）。S-绒毛能迅速降解ADP。当ADP与S-绒毛预孵育时，ADP诱导的血小板聚集完全消失。2. 胎盘BBMV具有很强的血小板聚集抑制活性。BBMV几乎完全抑制ADP、胶原、花生四烯酸和瑞斯托霉素诱导的血小板聚集。胎盘BBMV中还存在很强的ADP降解活性。BBMV能迅速降解ADP。当ADP与BBMV预孵育时，ADP诱导的血小板聚集完全消失。3. BBMV中ADP降解活性的酶学特性（热稳定性、酶动力学、Ca++依赖性和pH依赖性）与S-绒毛中的非常相似。4. S-绒毛和可溶性BBMV的ADP降解活性通过阴离子交换柱色谱和凝胶过滤柱色谱进行分级分离，结果显示二者的分级模式相似，且表明S-绒毛和可溶性BBMV的ADP降解物质的分子量约为60K。
