Iioka H, Hisanaga H, Moriyama I, Akada S, Morimoto K, Ichijo M
Department of Obstetrics and Gynecology, Nara Medical University.
Nihon Sanka Fujinka Gakkai Zasshi. 1990 Nov;42(11):1525-9.
We studied the platelet aggregation inhibiting activity of human placental syncytiotrophoblast brush border membrane vesicles (BBMV) and obtained the following results. 1. There existed a very strong platelet aggregation inhibiting activity in placental BBMV. The BBMV almost completely inhibited the platelet aggregation induced by ADP, arachidonic acid and ristocetin. 2. There existed a very strong ADP degrading activity (ADPase activity) in the placental BBMV. ADP was quickly decomposed to AMP by BBMV. 3. Placental BBMV inhibited platelet TXA2 (TXB2) production. In the 0.04 mg/ml protein concentration of BBMV, platelet TXA2 production was almost completely inhibited. 4. There existed a very strong platelet aggregation inhibiting activity in solved BBMV. Soluble BBMV almost completely inhibited the platelet aggregation induced by ADP and arachidonic acid but did not inhibit the platelet aggregation induced by ristocetin. 5. ADP decomposing activity and platelet TXA2 production inhibiting activity were separated by anion exchange column chromatography of soluble BBMV. 6. When BBMV was treated with ultrasonic waves to destroy the membrane structure, only the activity inhibiting ristocetin-induced platelet aggregation was markedly decreased.
我们研究了人胎盘合体滋养层刷状缘膜囊泡（BBMV）的血小板聚集抑制活性，并得到以下结果。1. 胎盘BBMV中存在很强的血小板聚集抑制活性。BBMV几乎完全抑制了由ADP、花生四烯酸和瑞斯托霉素诱导的血小板聚集。2. 胎盘BBMV中存在很强的ADP降解活性（ADP酶活性）。BBMV可将ADP迅速分解为AMP。3. 胎盘BBMV抑制血小板TXA2（TXB2）的产生。在BBMV蛋白浓度为0.04mg/ml时，血小板TXA2的产生几乎被完全抑制。4. 可溶性BBMV中存在很强的血小板聚集抑制活性。可溶性BBMV几乎完全抑制了由ADP和花生四烯酸诱导的血小板聚集，但不抑制由瑞斯托霉素诱导的血小板聚集。5. 通过可溶性BBMV的阴离子交换柱色谱法分离出ADP分解活性和血小板TXA2产生抑制活性。6. 当用超声波处理BBMV以破坏膜结构时，只有抑制瑞斯托霉素诱导的血小板聚集的活性明显降低。
