大豆倒伏及相关农艺性状的数量性状位点鉴定。

Identification of quantitative trait loci for lodging and related agronomic traits in soybean (Glycine max [L.] Merr.).

机构信息

The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, Guangdong, 510642, People's Republic of China.

The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, Guangdong, 510642, People's Republic of China.

出版信息

BMC Genomics. 2024 Sep 30;25(1):900. doi: 10.1186/s12864-024-10794-1.

DOI:10.1186/s12864-024-10794-1

PMID:39350068

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11440893/

Abstract

BACKGROUND

Lodging, a crucial agronomic trait linked to soybean yield, poses a significant challenge in soybean production. Nevertheless, there has been less research on soybean lodging compared to other important agronomic traits, hindering progress in breeding high-yield soybeans. Our goals were to investigate lodging, pinpoint quantitative trait loci (QTL) linked to lodging, and forecast potential candidate genes linked to this trait. To achieve this, we employed a recombinant inbred line (RIL) population derived from a cross between Guizao 1 and B13 (GB) across various environments.

RESULTS

The lodging score of the RIL population was found to be significantly positively correlated with flowering time, maturity time, plant height, number of main stem nodes, stem diameter, and internode length, with correlation coefficients ranging from 0.457 to 0.783. A total of 84 QTLs associated with soybean lodging and related traits were identified using the GB population. The contribution of phenotypic variance ranged from 1.26 to 66.87%, with LOD scores ranging from 2.52 to 69.22. Additionally, within these QTLs, a stable major QTL associated with lodging was newly discovered in the GB population. Out of the ten major QTLs associated with other related traits, nine of them were situated within the qLD-4-1 interval of the major lodging score locus, displaying phenotypic variations ranging from 12.10 to 66.87%. Specific alterations in gene expression were revealed through the analysis of resequencing data from the two parental lines, potentially indicating their significant roles in lodging. Subsequently, it was determined through qRT-PCR that four genes are likely to be the major genes controlling soybean lodging.

CONCLUSIONS

This study's findings offer valuable insights into the genetic underpinnings of soybean lodging resistance traits. By comprehending the potential genetic factors associated with lodging, this research lays the groundwork for breeding high-yield soybeans with improved lodging resistance.

摘要

背景

lodging 是一个与大豆产量密切相关的重要农艺性状，但相对于其他重要农艺性状，对大豆 lodging 的研究较少，这阻碍了高产大豆的培育进程。我们的目标是研究 lodging，确定与 lodging 相关的数量性状位点（QTL），并预测与该性状相关的潜在候选基因。为此，我们利用 Guizao 1 和 B13（GB）杂交衍生的重组自交系（RIL）群体，在不同环境下进行了研究。

结果

RIL 群体的 lodging 评分与开花时间、成熟时间、株高、主茎节数、茎直径和节间长度呈显著正相关，相关系数范围为 0.457 至 0.783。利用 GB 群体共鉴定到与大豆 lodging 及相关性状相关的 84 个 QTL。表型方差的贡献率范围为 1.26%至 66.87%，LOD 分数范围为 2.52%至 69.22%。此外，在这些 QTL 中，GB 群体中发现了一个与 lodging 相关的新的稳定主效 QTL。与其他相关性状相关的 10 个主效 QTL 中有 9 个位于主效 lodging 评分位点的 qLD-4-1 区间内，表型变异范围为 12.10%至 66.87%。通过对两个亲本系的重测序数据进行分析，揭示了特定基因表达的变化，这可能表明它们在 lodging 中具有重要作用。随后，通过 qRT-PCR 确定了四个可能控制大豆 lodging 的主效基因。