Chun JaeBuhm, Hwang Sadal
National Institute of Crop Science, Crop Foundation Research Division, Iseo-myeon, Wanju-Gun, Jeonbuk-do, Republic of Korea.
United States of America Department of Agriculture, Agricultural Research Service, Sam Farr United States of America Crop Improvement and Protection Research Center, Salinas, California, United States of America.
PLoS One. 2025 Mar 28;20(3):e0318815. doi: 10.1371/journal.pone.0318815. eCollection 2025.
As a domestication trait, pod dehiscence has a pleiotropic effect on agronomic traits and significantly contributes to yield loss in soybean. Population studies are still required to comprehend the genetic basis of dehiscence and to develop pod dehiscence-resistant cultivars with the optimal haplotype, thereby improving soybean production. We collected data for one wild (Glycine soja) (G. soja) and four cultivated (Glycine max) (G. max) populations from the USDA database. The G. max populations were evaluated in multi-environment conditions and used for genome-wide association study (GWAS) and selection. GWAS captured 86 quantitative trait loci (QTLs). Seventy-four new QTLs were colocalized in two different G. max populations, and 12 QTLs were closely mapped with previously reported QTLs. Eight out of 86 QTLs were associated with the domestication of pod dehiscence. We implemented marker-assisted selection (MAS) and genomic selection (GS) approaches to select pod dehiscence-resistant accessions with the best haplotype and lowest genomic breeding value (GBV), respectively. While our findings could be utilized for biology, genetics, and plant breeding, selecting pod dehiscence-resistant cultivars with the optimal haplotype will need further studies to confirm additional QTLs and assess advanced GS models.
作为一种驯化性状，豆荚开裂对农艺性状具有多效性，并显著导致大豆产量损失。仍需开展群体研究以了解豆荚开裂的遗传基础，并培育具有最佳单倍型的抗豆荚开裂品种，从而提高大豆产量。我们从美国农业部数据库中收集了一个野生大豆（野生大豆）和四个栽培大豆（栽培大豆）群体的数据。对栽培大豆群体在多环境条件下进行评估，并用于全基因组关联研究（GWAS）和选择。GWAS检测到86个数量性状位点（QTL）。74个新的QTL在两个不同的栽培大豆群体中定位，12个QTL与先前报道的QTL紧密连锁。86个QTL中有8个与豆荚开裂的驯化相关。我们分别采用标记辅助选择（MAS）和基因组选择（GS）方法，选择具有最佳单倍型和最低基因组育种值（GBV）的抗豆荚开裂材料。虽然我们的研究结果可用于生物学、遗传学和植物育种，但选择具有最佳单倍型的抗豆荚开裂品种还需要进一步研究，以确认更多的QTL并评估先进的GS模型。
