Kuleung C, Baenziger P S, Dweikat I
Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583-0915, USA.
Theor Appl Genet. 2004 Apr;108(6):1147-50. doi: 10.1007/s00122-003-1532-5. Epub 2003 Dec 5.
Simple sequence repeat (SSR) markers are a valuable tool for many purposes, such as mapping, fingerprinting, and breeding. However, they are only available in some economically important crops because of the high cost and labor intensity involved in their development. Comparative mapping reveals a high degree of colinearity between closely related species, which allows the exchange of markers between them. Our objective was to examine the transferability of SSR markers among wheat ( Triticum aestivum L.), rye ( Secale cereale L.), and triticale (X Triticosecale Wittmack). One hundred forty-eight wheat and 28 rye SSR markers were used to amplify genomic DNA extracted from five lines each of wheat, rye, and triticale. Transferability of wheat SSR markers to rye was 17%, whereas 25% of rye markers were amplifiable in wheat. In triticale, 58% and 39% transferability was achieved for wheat and rye markers, respectively. Wheat markers gave an average of 2.6, 2.7, and 2.4 polymorphic bands in wheat, rye, and triticale, respectively, while rye markers gave an average of 2.0 in rye and none in wheat and triticale. These transferable markers can now be exploited for further genetic and breeding studies in these species.
简单序列重复（SSR）标记是用于多种目的的有价值工具，如作图、指纹分析和育种。然而，由于其开发成本高且劳动强度大，它们仅在一些经济上重要的作物中可用。比较作图揭示了密切相关物种之间的高度共线性，这使得它们之间能够交换标记。我们的目标是研究SSR标记在小麦（普通小麦）、黑麦（黑麦草）和小黑麦（小黑麦属）之间的可转移性。使用148个小麦和28个黑麦SSR标记对从小麦、黑麦和小黑麦各5个品系中提取的基因组DNA进行扩增。小麦SSR标记对黑麦的可转移性为17%，而25%的黑麦标记可在小麦中扩增。在小黑麦中，小麦和黑麦标记的可转移性分别达到58%和39%。小麦标记在小麦、黑麦和小黑麦中分别平均产生2.6、2.7和2.4条多态性条带，而黑麦标记在黑麦中平均产生2.0条，在小麦和小黑麦中均无。这些可转移标记现在可用于这些物种的进一步遗传和育种研究。
