Mitrofanova I V, Galaev A V, Sivolap Iu M
Tsitol Genet. 2003 Nov-Dec;37(6):12-6.
Genome variability of in vitro micropropagated Clematis plants was established. The optimum concentrations of BAP and zeatin in the culture medium regulating in vitro morphogenetic processes in clematis explants cv. Serenada Kryma were determined. Molecular-genetic analysis of Clematis plants obtained via in vitro somatic embryogenesis and organogenesis was carried out. Using ISSR primers 105 amplicons have been revealed, six of them were polymorphic. The mean index of heterogeneity of clematis plants was 5.7%.
建立了体外微繁殖铁线莲属植物的基因组变异性。确定了在培养基中调节铁线莲属品种“Serenada Kryma”外植体体外形态发生过程的BAP和玉米素的最佳浓度。对通过体外体细胞胚胎发生和器官发生获得的铁线莲属植物进行了分子遗传学分析。使用ISSR引物揭示了105个扩增子，其中6个是多态性的。铁线莲属植物的平均异质性指数为5.7%。
