[利用限制性片段长度多态性（RFLP）和聚合酶链反应（PCR）方法对葡萄DNA品种内变异性的研究]

[Study of the intra-cultivar variability of grape DNA using RFLP and PCR methods].

作者信息

Barysheva I A, Tulaeva M I, Chisnikov V S

出版信息

Tsitol Genet. 2003 Nov-Dec;37(6):31-8.

Abstract

Evaluation of DNA polymorphism among Vitis vinifera varieties using RFLP and PCR methods has been performed to choose a DNA technology for detection of grape intracultivar variation. DNA polymorphism of clones of the varieties Riparia Gluar, Riparia x Rupestris 101-14, Cabernet Sauvignon, Riestling reinskiy has been studied using Southern hybridization and amplification techniques. It has been shown that grape intracultivar variability of rDNA in Riparia x Rupestris 101-14 and Cabernet Sauvignon clones was caused by the modification in Alul restriction sites of rDNA. DNA variability of the randomly amplified and inter-SSR sequences of the Riparia Gluar, Riparia x Rupestris 101-14, Cabernet Sauvignon clones was also detected. A set of molecular DNA loci which can be used for grape clone identification has been obtained.

摘要

利用限制性片段长度多态性（RFLP）和聚合酶链式反应（PCR）方法对葡萄品种间的DNA多态性进行了评估，以选择一种用于检测葡萄品种内变异的DNA技术。使用Southern杂交和扩增技术研究了河岸葡萄（Riparia Gluar）、河岸葡萄×沙地葡萄101-14（Riparia x Rupestris 101-14）、赤霞珠（Cabernet Sauvignon）、雷司令（Riestling reinskiy）品种克隆的DNA多态性。结果表明，河岸葡萄×沙地葡萄101-14和赤霞珠克隆中rDNA的葡萄品种内变异性是由rDNA的Alul限制性位点修饰引起的。还检测到了河岸葡萄、河岸葡萄×沙地葡萄101-14、赤霞珠克隆的随机扩增和简单序列重复区间（inter-SSR）序列的DNA变异性。获得了一组可用于葡萄克隆鉴定的分子DNA位点。