de Zeeuw R A, Franke J P, Dik E, ten Dolle W, Kam B L
Department of Analytical Chemistry and Toxicology, University Centre for Pharmacy, Groningen, The Netherlands.
J Forensic Sci. 1992 Jul;37(4):984-90.
The impact of high temperatures (24 to 39 degrees C) and low to moderately high humidities (20 to 70%) on the applicability of TLC systems for drug identification was studied during a 6 month climatologic cycle in Burkina Faso (West Africa). In general, the Rf values as observed on the plates were found to be substantially affected as compared with values obtained at temperate climates. Some TLC systems were more affected than others and the largest deviations of up to 30 Rf units were at low humidities. Tropical conditions also had a negative effect on the reproducibility of Rf values. However, when an Rf-correction procedure was applied, using reference mixtures of known drugs on each plate, accuracy as well as reproducibility of the resulting Rfc values were drastically improved and data thus corrected were found to be compatible with existing TLC data bases developed under moderate climatological conditions. The impact of high to extremely high humidities (70 to 100%) remains to be investigated.
在布基纳法索（西非）为期6个月的气候周期内，研究了高温（24至39摄氏度）和低至中高湿度（20至70%）对薄层色谱（TLC）系统用于药物鉴定适用性的影响。总体而言，与在温带气候下获得的值相比，发现板上观察到的比移值（Rf值）受到了显著影响。一些TLC系统比其他系统受影响更大，在低湿度下最大偏差可达30个Rf单位。热带条件对Rf值的重现性也有负面影响。然而，当应用Rf校正程序时，即在每个板上使用已知药物的参考混合物，所得Rfc值的准确性和重现性都得到了显著提高，并且发现这样校正后的数据与在适度气候条件下建立的现有TLC数据库兼容。高至极高湿度（70至100%）的影响仍有待研究。
