de Zeeuw R A, Franke J P, van Halem M, Schaapman S, Logawa E, Siregar C J
Department of Analytical Chemistry and Toxicology, University Centre for Pharmacy, Groningen, The Netherlands.
J Anal Toxicol. 1994 Nov-Dec;18(7):402-6. doi: 10.1093/jat/18.7.402.
Because thin-layer chromatography can be affected by climatic conditions, we investigated the impact of high temperatures (33-38 degrees C) and high relative humidities (80-100%) on the applicability of Toxi-Lab thin-layer chromatographic systems for the screening of basic and neutral drugs during a 6-month climatological cycle in Jakarta, Indonesia that can be characterized as hot and humid. In general, the Rf values as observed on the plate showed substantial deviations from those in the literature obtained in moderate climates. Most substances gave higher Rf values, and the deviations (sometimes amounting to 20 Rf units) increased with increasing humidities. However, when the observed Rf values were corrected by means of a reference mixture of standard drugs that was run on each plate, much better accuracy and reproducibility were obtained. Thus, these corrected Rcf values were compatible with the existing databases developed in temperate climates. These results are in line with earlier studies carried out under hot and dry conditions. In the earlier studies, the observed Rf values tended to be lower than those present in other databases, but here too, the Rf correction procedure corrected this phenomenon.
由于薄层色谱会受到气候条件的影响，我们在印度尼西亚雅加达一个为期6个月的气候周期内，研究了高温（33 - 38摄氏度）和高相对湿度（80 - 100%）对Toxi-Lab薄层色谱系统筛查碱性和中性药物适用性的影响。雅加达的气候特点是炎热潮湿。总体而言，平板上观察到的比移值（Rf值）与在温和气候条件下文献中报道的值存在显著偏差。大多数物质的Rf值更高，且偏差（有时高达20个Rf单位）随湿度增加而增大。然而，当通过在每块平板上运行标准药物的参比混合物来校正观察到的Rf值时，可获得更高的准确性和重现性。因此，这些校正后的Rcf值与在温带气候条件下建立的现有数据库相符。这些结果与早期在炎热干燥条件下进行的研究一致。在早期研究中，观察到的Rf值往往低于其他数据库中的值，但在此处同样，Rf校正程序纠正了这一现象。
