Parodi B, Caviglioli G, Bachi A, Cafaggi S, Romussi G
Istituto di Analisi e Tecnologie Farmaceutiche ed Alimentari, Genoa University, Italy.
Pharmazie. 1993 Sep;48(9):678-81.
A method for the detection and quantitation of several undeclared drugs in herbal preparations with slimming activity is proposed. Samples containing various anorexics, hypoglycemics and antidepressants were prepared by addition of the drugs to a synthetic mixture containing the most commonly used plant powders for those preparations. Each sample was subjected to a treatment that permitted, after a simple ethanolic extraction, the identification of the drugs by TLC using three different solvent systems. A further purification of the ethanolic solution through a polyamide column allowed for quantitative analysis of the drugs by a RP-HPLC method. The analytical recovery was good (88-97%); the calibration curves were linear over a wide range of drug concentrations (30-500 micrograms/ml) (r > 0.9995); the precision was high (CV% = 0.4-2.8) as well as the accuracy (96-102%).
提出了一种检测和定量测定具有减肥活性的草药制剂中几种未申报药物的方法。通过将药物添加到含有这些制剂中最常用植物粉末的合成混合物中来制备含有各种食欲抑制剂、降血糖药和抗抑郁药的样品。每个样品经过一种处理，在简单的乙醇提取后，使用三种不同的溶剂系统通过薄层色谱法鉴定药物。通过聚酰胺柱对乙醇溶液进行进一步纯化，以便通过反相高效液相色谱法对药物进行定量分析。分析回收率良好（88-97%）；校准曲线在很宽的药物浓度范围（30-500微克/毫升）内呈线性（r>0.9995）；精密度高（变异系数%=0.4-2.8），准确度也高（96-102%）。
