分化型早期胃癌及具有胃细胞表型的背景黏膜中syndecan-1表达的降低

Reduction of syndecan-1 expression in differentiated type early gastric cancer and background mucosa with gastric cellular phenotype.

作者信息

Watari Jiro, Saitoh Yusuke, Fujiya Mikihiro, Shibata Naomi, Tanabe Hiroki, Inaba Yuhei, Okamoto Kotaro, Maemoto Atsuo, Ohta Tomoyuki, Yasuda Atsumi, Ayabe Tokiyoshi, Ashida Toshifumi, Yokota Kinichi, Obara Takeshi, Kohgo Yutaka

机构信息

Third Department of Internal Medicine, Asahikawa Medical College, 2-1-1-1 Midorigaoka-Higashi, 078-8510, Asahikawa, Japan.

出版信息

J Gastroenterol. 2004;39(2):104-12. doi: 10.1007/s00535-003-1260-2.

DOI:10.1007/s00535-003-1260-2

PMID:15069616

Abstract

BACKGROUND

Syndecan-1 is known to play a role as a cell adhesion molecule, similar to E-cadherin, and is associated with the maintenance of epithelial morphology. The purpose of this study was to elucidate the role and alterations of syndecan-1 expression in comparison with those of E-cadherin in different cellular phenotypes of differentiated-type gastric cancers (DGCs).

METHODS

A total of 80 DGCs at an early stage, and their adjacent mucosa, were evaluated by both immunohistochemistry and in situ hybridization. Syndecan-1 and E-cadherin were assessed by immunohistochemical staining with an anti-syndecan-1 and an anti-E-cadherin antibody, respectively. Based on immunohistochemistry, DGCs and their surrounding mucosa were divided into four types: gastric type (G-type), ordinary type (O-type), complete-intestinal type (CI-type), and null type.

RESULTS

The expression sites of syndecan-1 mRNA mostly coincided with those of syndecan-1 protein. Syndecan-1 expression was significantly lower in G-type cancers (30%) than in O- (81%) and CI-type cancers (92%) ( P = 0.0001 and P = 0.004, respectively), but E-cadherin did not show this result. In addition, syndecan-1 expression was significantly reduced in DGCs comprised partly of poorly differentiated adenocarcinoma or signet-ring cell carcinoma, compared to DGCs demonstrating papillary and/or tubular adenocarcinoma ( P = 0.02). G-type intestinal metaplasia (IM) surrounding the tumors was observed in 21% of G-type cancers, in 0% of O-, and in 10% of CI-type cancers ( P = 0.01; G-type vs O-type). Reduction of syndecan-1 expression was significant in G-type IM (25%) compared to non-G-type IM (75%; P = 0.02).

CONCLUSIONS

Syndecan-1 plays a role in the growth of G-type cancers at an early stage compared with E-cadherin changes, and the reduction of syndecan-1 expression in IM surrounding the tumors may influence the growth of G-type cancer.

摘要

背景

已知Syndecan-1作为一种细胞粘附分子发挥作用，类似于E-钙粘蛋白，并与上皮形态的维持有关。本研究的目的是阐明Syndecan-1表达在分化型胃癌（DGC）不同细胞表型中的作用及变化，并与E-钙粘蛋白进行比较。

方法

通过免疫组织化学和原位杂交对80例早期DGC及其邻近黏膜进行评估。分别用抗Syndecan-1抗体和抗E-钙粘蛋白抗体通过免疫组织化学染色评估Syndecan-1和E-钙粘蛋白。基于免疫组织化学，将DGC及其周围黏膜分为四种类型：胃型（G型）、普通型（O型）、完全肠型（CI型）和无表达型。

结果

Syndecan-1 mRNA的表达位点大多与Syndecan-1蛋白的表达位点一致。Syndecan-1在G型癌（30%）中的表达显著低于O型癌（81%）和CI型癌（92%）（P分别为0.0001和0.004），但E-钙粘蛋白未显示此结果。此外，与表现为乳头状和/或管状腺癌的DGC相比，部分由低分化腺癌或印戒细胞癌组成的DGC中Syndecan-1表达显著降低（P = 0.02）。肿瘤周围的G型肠化生（IM）在21%的G型癌中观察到，在0%的O型癌和10%的CI型癌中观察到（P = 0.01；G型与O型）。与非G型IM（75%）相比，G型IM中Syndecan-1表达的降低显著（P = 0.02）。