Shimoda Hiroshi, Takahashi Yoshiaki, Kato Seiji
Department of Anatomy, Biology and Medicine, Faculty of Medicine, Oita University, 1-1 Idaigaoka, 879-5593, Oita, Japan.
Cell Tissue Res. 2004 Jun;316(3):325-38. doi: 10.1007/s00441-004-0889-3. Epub 2004 Apr 7.
Regeneration of lymphatic vessels after transection of the muscle coat in the rat jejunum was studied by histochemical methods. The lymphatic regrowth occurred behind the regeneration of blood vessels. Enzyme histochemistry for 5'-nucleotidase (5'-Nase) demonstrated the manner of lymphatic regrowth, which was essentially attributed to vascular sprouting from preexisting lymphatics, and structural changes of the endothelial cells indicating their high migratory potential. The lymphatic regeneration progressively advanced with vascular maturation throughout the experimental period. The expression of 5'-Nase in the regenerating lymphatics was increased in proportion to their growth. VEGF-C, a highly specific lymphangiogenic factor, was highly expressed in a subpopulation of interstitial cells, being close to the regrowing lymphatics with immunoreactivity of its receptor, VEGFR-3, in the regenerative area. The present findings suggest that transection of the intestinal muscle coat affords a useful experimental model for the investigation of lymphatic regeneration in tissue repair and that the interstitium may play a crucial role in lymphangiogenesis.
采用组织化学方法研究了大鼠空肠肌层横断后淋巴管的再生情况。淋巴管的再生发生在血管再生之后。5'-核苷酸酶（5'-Nase）的酶组织化学显示了淋巴管再生的方式，其主要归因于原有淋巴管的血管芽生，以及内皮细胞的结构变化，表明其具有较高的迁移潜能。在整个实验期间，随着血管成熟，淋巴管再生逐渐推进。再生淋巴管中5'-Nase的表达随其生长而增加。血管内皮生长因子C（VEGF-C）是一种高度特异性的淋巴管生成因子，在间质细胞亚群中高表达，在再生区域靠近再生淋巴管，且具有其受体VEGFR-3的免疫反应性。目前的研究结果表明，肠肌层横断为组织修复中淋巴管再生的研究提供了一个有用的实验模型，并且间质可能在淋巴管生成中起关键作用。
