Ko Jae-Hong, Asano Atsushi, Kon Yasuhiro, Watanabe Tomomasa, Agui Takashi
Laboratory of Experimental Animal Science, Department of Disease Control Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Jpn J Vet Res. 2004 Feb;51(3-4):123-33.
The double-stranded RNA-dependent protein kinase (PKR) is induced in mouse and human cells on treatment with interferon. In this study, we have cloned and determined the nucleotide sequences of chicken PKR cDNA in various chicken breeds. Chicken PKR was a 550-amino-acid protein as deduced from the cDNA open reading frame (ORF), and there were specific domains (two double-stranded RNA binding domains (DRBDs) and numerous kinase subdomains) characterized in RNA binding proteins and kinase families. Furthermore, it was suggested that chicken PKR was polymorphic. Transfected cell clones expressing chicken PKR mRNA were demonstrated to confer antiviral responses to vesicular stomatitis virus, except for Koshamo type-3 (KS-3). KS-3 PKR, which has an amino acid substitution at position 507 (Arg to Gln), showed amphibious antiviral responses. This specific amino acid substitution was considered to determine the antiviral function of chicken PKR in addition to essential domains as DRBDs and kinase subdomains.
双链RNA依赖性蛋白激酶（PKR）在小鼠和人类细胞中经干扰素处理后被诱导产生。在本研究中，我们克隆并测定了多种鸡品种中鸡PKR cDNA的核苷酸序列。从cDNA开放阅读框（ORF）推导，鸡PKR是一种含550个氨基酸的蛋白质，并且存在RNA结合蛋白和激酶家族所特有的特定结构域（两个双链RNA结合结构域（DRBDs）和众多激酶亚结构域）。此外，提示鸡PKR具有多态性。除了科沙莫3型（KS - 3）外，表达鸡PKR mRNA的转染细胞克隆被证明可赋予对水疱性口炎病毒的抗病毒反应。在第507位氨基酸发生取代（从精氨酸到谷氨酰胺）的KS - 3 PKR表现出两栖抗病毒反应。除了作为DRBDs和激酶亚结构域的必需结构域外，这种特定的氨基酸取代被认为决定了鸡PKR的抗病毒功能。
