Zha Wenjuan, Shao Zengyi, Frost John W, Zhao Huimin
Department of Chemical and Biomolecular Engineering, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
J Am Chem Soc. 2004 Apr 14;126(14):4534-5. doi: 10.1021/ja0317271.
Metabolic pathway engineering is a powerful tool to synthesize structurally diverse and complex chemicals via genetic manipulation of multistep catalytic systems involved in cell metabolism. Here, we report the rational design of a fatty acid biosynthetic pathway, Brevibacterium ammoniagenes fatty acid synthase B (FAS-B), that allows the microbial synthesis of triacetic acid lactone (TAL) from an inexpensive feedstock, d-glucose. TAL can be chemically converted to phloroglucinol, which is a core structure for the synthesis of various high value bioactive compounds and energetic compounds such as 1,3,5-triamino-2,4,6-trinitrobenzene (TATB). Synthesis of phloroglucinol from d-glucose using this combined biological and chemical synthesis may offer significant advantages over the current phloroglucinol manufacture, including environmental friendliness and reduction in the cost of phloroglucinol. More importantly, it represents a novel strategy for the benzene-free synthesis of aromatic chemicals.
代谢途径工程是一种强大的工具，可通过对细胞代谢中涉及的多步催化系统进行基因操作来合成结构多样且复杂的化学品。在此，我们报告了一种脂肪酸生物合成途径——产氨短杆菌脂肪酸合酶B（FAS-B）的合理设计，该途径可使微生物从廉价原料d-葡萄糖合成三乙酸内酯（TAL）。TAL可通过化学方法转化为间苯三酚，间苯三酚是合成各种高价值生物活性化合物和高能化合物（如1,3,5-三氨基-2,4,6-三硝基苯（TATB））的核心结构。利用这种生物和化学合成相结合的方法从d-葡萄糖合成间苯三酚，可能比目前的间苯三酚生产具有显著优势，包括环境友好性和间苯三酚成本的降低。更重要的是，它代表了一种无苯合成芳香族化学品的新策略。
