工程化脂肪酸合酶以定向聚酮化合物的生产。
Engineering fatty acid synthases for directed polyketide production.
机构信息
Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Cluster of Excellence "Macromolecular Complexes," Goethe University Frankfurt, Frankfurt am Main, Germany.
Max Planck Institute for Biophysical Chemistry, Department of Theoretical and Computational Biophysics, Göttingen, Germany.
出版信息
Nat Chem Biol. 2017 Apr;13(4):363-365. doi: 10.1038/nchembio.2314. Epub 2017 Feb 20.
In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids and polyketides, guided by a combined in vitro and in silico approach. Along with exploring the synthetic capability of FAS, we aim to build a foundation for efficient protein engineering, with the specific goal of harnessing evolutionarily related megadalton-scale polyketide synthases (PKS) for the tailored production of bioactive natural compounds.
在这项研究中，我们通过体外和计算机模拟相结合的方法对脂肪酸合酶（FAS）进行工程改造，用于短链脂肪酸和聚酮化合物的生物合成。除了探索 FAS 的合成能力外，我们还旨在为高效蛋白质工程奠定基础，具体目标是利用进化上相关的兆道尔顿级聚酮合酶（PKS）来定制生产生物活性天然化合物。
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