Shanmugasundaram Natesan, Ravikumar Thangappan, Babu Mary
Department of Biomaterials, Central Leather Research Institute, Adyar, Chennai 600 020, India.
J Biomater Appl. 2004 Apr;18(4):247-64. doi: 10.1177/0885328204040945.
Collagen from different sources was isolated and designed as scaffolds to act as a three-dimensional substrate for culturing human skin fibroblasts, which can be used as dermal substitutes. The thermodynamic behavior of the scaffolds developed was analyzed through Differential Scanning Calorimetric (DSC) and Thermogravimetric analysis (TGA). Analysis by Fourier Transform Infrared Spectroscopy (FTIR) revealed the functional groups in the scaffolds and the mechanical stability of various scaffolds was assessed through tensile strength analysis. Human skin fibroblasts were cultured on the developed scaffolds to assess their cellular interaction and behavior, and the morphological characteristics of the cultured fibroblasts were evaluated using Scanning Electron Microscopy (SEM). The collagen scaffold exhibited unique features when developed from various sources and it was observed that cells could grow and proliferate well and spread as a monolayer in the reconstituted collagen scaffold.
从不同来源分离出胶原蛋白，并将其设计成支架，作为培养人类皮肤成纤维细胞的三维基质，这些支架可用作真皮替代物。通过差示扫描量热法（DSC）和热重分析（TGA）分析所开发支架的热力学行为。傅里叶变换红外光谱（FTIR）分析揭示了支架中的官能团，并通过拉伸强度分析评估了各种支架的机械稳定性。将人类皮肤成纤维细胞培养在所开发的支架上，以评估它们的细胞相互作用和行为，并使用扫描电子显微镜（SEM）评估培养的成纤维细胞的形态特征。当从各种来源开发时，胶原蛋白支架表现出独特的特性，并且观察到细胞可以在重组胶原蛋白支架中良好地生长和增殖，并单层铺展。
