Chen Cheng, Jiang Yan, Kan Jinqing
School of Chemistry and Chemical Engineering, Yangzhou University,Yangzhou 225002, PR China.
Biosens Bioelectron. 2006 Dec 15;22(5):639-43. doi: 10.1016/j.bios.2006.01.023. Epub 2006 Mar 15.
In order to eliminate the interference of impurities, such as ascorbic acid, a noninterference polypyrrole glucose biosensor was constructed with a four-electrode cell consisting of a polypyrrole film electrode, a polypyrrole-glucose oxidase electrode, a counter electrode and a reference electrode. The pure catalytic current of glucose oxidase (GOD) can be obtained from the difference between response currents of two working electrodes with and without GOD. The effects of potential, pH and temperature on analytical performance of the glucose biosensor were discussed. The optimum pH and apparent activation energy of enzyme-catalyzed reaction are 5.5 and 25 kJ mol(-1), respectively. The response current of the biosensor increases linearly with the increasing glucose concentration from 0.005 to 20.0 mmol dm(-3). The results show the glucose biosensor with under 2% of relative deviation has good ability of anti-interference. The glucose biosensor was also characterized with FT-IR and UV-vis spectra.
为消除抗坏血酸等杂质的干扰，构建了一种无干扰的聚吡咯葡萄糖生物传感器，该传感器采用四电极池，由聚吡咯膜电极、聚吡咯-葡萄糖氧化酶电极、对电极和参比电极组成。葡萄糖氧化酶（GOD）的纯催化电流可通过有GOD和无GOD的两个工作电极的响应电流之差获得。讨论了电位、pH值和温度对葡萄糖生物传感器分析性能的影响。酶催化反应的最佳pH值和表观活化能分别为5.5和25 kJ mol(-1)。生物传感器的响应电流随葡萄糖浓度从0.005增加到20.0 mmol dm(-3)呈线性增加。结果表明，相对偏差低于2%的葡萄糖生物传感器具有良好的抗干扰能力。还通过傅里叶变换红外光谱（FT-IR）和紫外可见光谱对葡萄糖生物传感器进行了表征。
