Denkbaş Emir B, Oztürk Eylem, Ozdemir Nalan, Keçeci Kaan, Agalar Canan
Chemistry Department, Biochemistry Division Hacettepe University, Ankara, Turkey.
J Biomater Appl. 2004 Apr;18(4):291-303. doi: 10.1177/0885328204041510.
The aim of this study was the preparation and characterization of chitosan sponges including a model antibiotic (i.e., norfloxacin). The chitosan sponges were prepared by a solvent evaporation method. The matrix was also cross-linked during the preparation. The results indicated that the chitosan sponges were in the fibrillar structure. The swelling behavior, norfloxacin loading, in vitro release characteristics, and antibacterial activity were determined. The effects of cross-linker concentration, norfloxacin/chitosan ratio, chitosan molecular weight, and base concentration were investigated. The most effective parameter was found to be the degree of neutralization. It was also observed that the equilibrium swelling ratio decreased with increasing cross-linking density. The norfloxacin release was found to be swelling controlled initially and diffusion controlled at the extended release periods. It was also found that the antibacterial activity was directly proportional to the release rate.
本研究的目的是制备并表征包含模型抗生素（即诺氟沙星）的壳聚糖海绵。壳聚糖海绵通过溶剂蒸发法制备。在制备过程中基质也进行了交联。结果表明壳聚糖海绵呈纤维状结构。测定了其溶胀行为、诺氟沙星负载量、体外释放特性及抗菌活性。研究了交联剂浓度、诺氟沙星/壳聚糖比例、壳聚糖分子量和碱浓度的影响。发现最有效的参数是中和度。还观察到平衡溶胀率随交联密度的增加而降低。发现诺氟沙星的释放最初受溶胀控制，在延长释放期受扩散控制。还发现抗菌活性与释放速率成正比。
