RNA聚合酶σ亚基在不依赖启动子的转录起始中的作用。
The role of RNA polymerase sigma subunit in promoter-independent initiation of transcription.
作者信息
Zenkin Nikolay, Severinov Konstantin
机构信息
The Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
出版信息
Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4396-400. doi: 10.1073/pnas.0400886101. Epub 2004 Mar 15.
Abstract
In bacteria, initiation of transcription depends on the RNA polymerase sigma subunit, which brings catalytically proficient RNA polymerase core to promoters by binding to specific DNA elements located upstream of the transcription start point. Here, we study sigma-dependent synthesis of a transcript that is used to prime replication of the single-stranded genome of bacteriophage M13. We show that, in this system, sigma plays no role in DNA recognition, which is accomplished solely through RNA polymerase core interaction with DNA downstream of the transcription start point. However, sigma is required for full-sized transcript synthesis by allowing RNA polymerase core to escape into productive elongation. RNA polymerase sigma may play a similar role during replication primer synthesis in other bacterial mobile elements whose life cycle involves a single-stranded DNA stage.
摘要
在细菌中，转录起始依赖于RNA聚合酶的σ亚基，该亚基通过与位于转录起始点上游的特定DNA元件结合，将具有催化活性的RNA聚合酶核心带到启动子上。在这里，我们研究了用于引发噬菌体M13单链基因组复制的转录本的σ依赖性合成。我们表明，在这个系统中，σ在DNA识别中不起作用，DNA识别仅通过RNA聚合酶核心与转录起始点下游的DNA相互作用来完成。然而，σ对于全长转录本的合成是必需的，它允许RNA聚合酶核心进入有效的延伸阶段。在其他生命周期涉及单链DNA阶段的细菌移动元件的复制引物合成过程中，RNA聚合酶σ可能发挥类似作用。
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