Niedrig Matthias, Schmitz Herbert, Becker Stephan, Günther Stephan, ter Meulen Jan, Meyer Herman, Ellerbrok Heinz, Nitsche Andreas, Gelderblom Hans R, Drosten Christian
Robert Koch-Institute, Berlin, Germany.
J Clin Microbiol. 2004 Apr;42(4):1753-5. doi: 10.1128/JCM.42.4.1753-1755.2004.
We have conducted an international quality assurance study of filovirus, Lassa virus, and orthopox virus PCR with 24 participants. Of the participating laboratories, 45.8 and 66.7% detected virus in all plasma samples, which contained > or = 5,000 and > or = 100,000 copies per ml, respectively. Sensitivity levels were not significantly different between viruses. False-negative results were attributable to a lack of sensitivity.
我们对24名参与者进行了一项关于丝状病毒、拉沙病毒和正痘病毒PCR的国际质量保证研究。在参与研究的实验室中，分别有45.8%和66.7%的实验室在所有血浆样本中检测到了病毒，这些样本每毫升分别含有≥5000和≥100000个拷贝。不同病毒之间的灵敏度水平没有显著差异。假阴性结果归因于灵敏度不足。
