首次关于生物恐怖主义病毒病原体快速检测的国际质量保证研究。
First international quality assurance study on the rapid detection of viral agents of bioterrorism.
作者信息
Niedrig Matthias, Schmitz Herbert, Becker Stephan, Günther Stephan, ter Meulen Jan, Meyer Herman, Ellerbrok Heinz, Nitsche Andreas, Gelderblom Hans R, Drosten Christian
机构信息
Robert Koch-Institute, Berlin, Germany.
出版信息
J Clin Microbiol. 2004 Apr;42(4):1753-5. doi: 10.1128/JCM.42.4.1753-1755.2004.
Abstract
We have conducted an international quality assurance study of filovirus, Lassa virus, and orthopox virus PCR with 24 participants. Of the participating laboratories, 45.8 and 66.7% detected virus in all plasma samples, which contained > or = 5,000 and > or = 100,000 copies per ml, respectively. Sensitivity levels were not significantly different between viruses. False-negative results were attributable to a lack of sensitivity.
摘要
我们对24名参与者进行了一项关于丝状病毒、拉沙病毒和正痘病毒PCR的国际质量保证研究。在参与研究的实验室中，分别有45.8%和66.7%的实验室在所有血浆样本中检测到了病毒，这些样本每毫升分别含有≥5000和≥100000个拷贝。不同病毒之间的灵敏度水平没有显著差异。假阴性结果归因于灵敏度不足。
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