Ellerbrok Heinz, Jacobsen Sonja, Patel Pranav, Rieger Toni, Eickmann Markus, Becker Stephan, Günther Stephan, Naidoo Dhamari, Schrick Livia, Keeren Kathrin, Targosz Angelina, Teichmann Anette, Formenty Pierre, Niedrig Matthias
Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany.
Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
PLoS Negl Trop Dis. 2017 May 1;11(5):e0005570. doi: 10.1371/journal.pntd.0005570. eCollection 2017 May.
During the recent Ebola outbreak in West Africa several international mobile laboratories were deployed to the mainly affected countries Guinea, Sierra Leone and Liberia to provide ebolavirus diagnostic capacity. Additionally, imported cases and small outbreaks in other countries required global preparedness for Ebola diagnostics. Detection of viral RNA by reverse transcription polymerase chain reaction has proven effective for diagnosis of ebolavirus disease and several assays are available. However, reliability of these assays is largely unknown and requires serious evaluation. Therefore, a proficiency test panel of 11 samples was generated and distributed on a global scale. Panels were analyzed by 83 expert laboratories and 106 data sets were returned. From these 78 results were rated optimal and 3 acceptable, 25 indicated need for improvement. While performance of the laboratories deployed to West Africa was superior to the overall performance there was no significant difference between the different assays applied.
在近期西非埃博拉疫情爆发期间，多个国际移动实验室被部署到主要受灾国几内亚、塞拉利昂和利比里亚，以提供埃博拉病毒诊断能力。此外，其他国家的输入性病例和小规模疫情爆发需要全球具备埃博拉诊断的准备。通过逆转录聚合酶链反应检测病毒RNA已被证明对埃博拉病毒病的诊断有效，并且有几种检测方法可用。然而，这些检测方法的可靠性很大程度上未知，需要进行认真评估。因此，生成了一个包含11个样本的能力验证检测组并在全球范围内分发。83个专业实验室对检测组进行了分析，并返回了106个数据集。其中78个结果被评为最佳，3个可接受，25个表明需要改进。虽然部署到西非的实验室的表现优于总体表现，但所应用的不同检测方法之间没有显著差异。
