Ndao M, Magnus E, Büscher P, Geerts S
Institute of Tropical Medicine, Department of Parasitology, Nationalestraat 155, B-200 Antwerp, Belgium.
Parasite. 2004 Mar;11(1):103-6. doi: 10.1051/parasite/2004111103.
A rodent adapted clone of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll solutions, 1.07, 1.06, 1.05 g/ml, respectively, carefully layered on top of each other. The yields of this simple procedure for trypanosome purification were about six times higher than those obtained with the conventional anion-exchange columns. Cryopreservation of trypanosomes using glycerol yielded 90% viable parasites, whereas using dimethylsulfoxide, a more commonly used cryoprotectant, the viability was only 35%.
使用一种适应啮齿动物的间日锥虫克隆株感染经环磷酰胺处理的小鼠和大鼠。含有锥虫的新鲜血液在分别为1.07、1.06、1.05 g/ml的三种 Percoll 溶液的密度梯度中进行离心，这些溶液小心地彼此层叠放置。这种用于锥虫纯化的简单方法的产量比使用传统阴离子交换柱获得的产量高约六倍。使用甘油对锥虫进行冷冻保存可产生90%的活寄生虫，而使用更常用的冷冻保护剂二甲基亚砜时，存活率仅为35%。
