González L E, García J A, Núñez C, Perrone T M, González-Baradat B, Gonzatti M I, Reyna-Bello A
Universidad Simón Bolívar, Departamento de Biología Celular, Grupo deBioquímica e Inmunología de Hemoparásitos, Caracas, Venezuela.
Exp Parasitol. 2005 Oct;111(2):126-9. doi: 10.1016/j.exppara.2005.05.008.
Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.
活跃锥虫是牛锥虫病的主要病原体，这是一种在热带和亚热带地区广泛传播的疾病。在此，我们提出了一种简单且可重复的方法，用于从实验感染并免疫抑制的绵羊中纯化活跃锥虫，该方法采用等密度Percoll梯度离心，随后进行DEAE-纤维素色谱法，估计产量为11%-15%。此方法可用于从不同地点和不同自然宿主中纯化活跃锥虫的地理分离株。
