Xiong Lin, Dai Ya-ling
Department of Dermato-venereology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Mar;35(2):277-9.
To assess the value of methylene blue and parker ink staining in the detection of Malassezia.
Detecting Malassezia in three dermatoses samples (pityriasis versicolor, Malassezia folliculitis and dandruff) stained by methylene blue (3 g/L) or parker ink. Comparing the effectiveness and the positive rates of Malassezia detection between the two staining methods. Staining Malassezia and Candida and comparing the specificity of the two staining methods in staining Malassezia.
Dark blue hyphae and spores were observed in smears stained by methylene blue, and light blue hyphae and spores were observed in smears stained by parker ink. Hyphae and spores were easy to identify on the background. The positive rates of methylene blue and parker ink for detecting Malassezia in the three dermatoses were 96% and 94% respectively. There was no significant difference between the two dye liquors in the positive rate for deterting Malassezia. Both Malassezia and Candida could be stained by methylene blue; nevertheless, only Malassezia could be stained by parker ink.
Both methylene blue and parker ink staining can be used to detect Malassezia, but parker ink has higher specificity in Malassezia staining.
评估亚甲蓝和派克墨水染色法在检测马拉色菌中的价值。
用亚甲蓝（3 g/L）或派克墨水对三种皮肤病样本（花斑癣、马拉色菌毛囊炎和头皮屑）进行染色以检测马拉色菌。比较两种染色方法检测马拉色菌的有效性和阳性率。对马拉色菌和念珠菌进行染色，比较两种染色方法对马拉色菌染色的特异性。
亚甲蓝染色涂片可见深蓝色菌丝和孢子，派克墨水染色涂片可见浅蓝色菌丝和孢子。菌丝和孢子在背景上易于识别。亚甲蓝和派克墨水检测三种皮肤病中马拉色菌的阳性率分别为96%和94%。两种染液检测马拉色菌的阳性率无显著差异。亚甲蓝可对马拉色菌和念珠菌进行染色；然而派克墨水仅可对马拉色菌进行染色。
亚甲蓝和派克墨水染色均可用于检测马拉色菌，但派克墨水对马拉色菌染色具有更高的特异性。
