Gaitanis G, Velegraki A, Alexopoulos E C, Chasapi V, Tsigonia A, Katsambas A
Mycology Laboratory, Microbiology Department, Medical School, University of Athens, and 3rd Dermatology Department, A. Sygros Hospital, Athens, Greece.
Br J Dermatol. 2006 May;154(5):854-9. doi: 10.1111/j.1365-2133.2005.07114.x.
The expansion of the genus Malassezia has generated interest in the epidemiological investigation of the distribution of new species in a range of dermatoses, on which variable results have been reported from different geographical regions. No data are thus far available from South-east Europe (Greece).
To study the distribution of Malassezia species in pityriasis versicolor (PV) and seborrhoeic dermatitis (SD) and to investigate whether polymorphisms in the internal transcribed spacer (ITS) 1 region facilitate detection of M. globosa and M. sympodialis subtypes.
In total, 109 patients with PV and SD and positive Malassezia cultures were included in the study. Age, gender, primary/recurrent episode, disease extent and clinical form of PV were recorded. ITS 1 polymorphisms of M. globosa and M. sympodialis type and clinical strains were investigated by polymerase chain reaction (PCR)-single-strand conformational polymorphism (SSCP) analysis.
Malassezia globosa was the prevalent species isolated from PV and SD either alone (77% and 39%, respectively) or in combination (13% and 18%, respectively) with other Malassezia species. The pigmented form of PV was strongly correlated with the female gender. PCR-SSCP differentiated five subgroups of M. globosa with one being associated with extensive clinical disease. All M. sympodialis isolates displayed a homogeneous ITS 1 PCR-SSCP profile.
Malassezia species isolation rates were in agreement with those reported from South-west Europe. PCR-SSCP of the ITS 1 is useful for highlighting prospective clinical implications of M. globosa subtypes.
马拉色菌属的扩展引发了人们对一系列皮肤病中新物种分布进行流行病学调查的兴趣，不同地理区域报告的结果各不相同。迄今为止，东南欧（希腊）尚无相关数据。
研究马拉色菌在花斑糠疹（PV）和脂溢性皮炎（SD）中的分布，并调查内部转录间隔区（ITS）1区域的多态性是否有助于检测球形马拉色菌和合轴马拉色菌亚型。
本研究共纳入109例PV和SD患者，其马拉色菌培养结果为阳性。记录患者的年龄、性别、初次/复发情况、疾病范围以及PV的临床类型。通过聚合酶链反应（PCR）-单链构象多态性（SSCP）分析研究球形马拉色菌和合轴马拉色菌型及临床菌株的ITS 1多态性。
球形马拉色菌是从PV和SD中单独分离出的优势菌种（分别为77%和39%），或与其他马拉色菌种联合分离出的优势菌种（分别为13%和18%）。PV的色素沉着型与女性密切相关。PCR-SSCP区分出球形马拉色菌的五个亚组，其中一个亚组与广泛的临床疾病相关。所有合轴马拉色菌分离株均显示出一致的ITS 1 PCR-SSCP图谱。
马拉色菌种的分离率与西南欧报告的结果一致。ITS 1的PCR-SSCP有助于突出球形马拉色菌亚型的潜在临床意义。
