Etson Cathy J, Waldner Cheryl L, Barth Albert D
Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4.
Can Vet J. 2004 Mar;45(3):235-40.
Changes in concentrations of cortisol and progesterone in serial blood samples were used to quantify a stress response to different methods of electroejaculation in 10 Hereford bulls. Treatments included restraint (control), and electroejaculation using rectal probes with segmented electrodes or conventional nonsegmented electrodes, with or without lidocaine caudal epidural anesthesia. A subjective scoring system was used to assess behavioral responses to the different methods of electroejaculation. The increases in concentrations of serum cortisol and progesterone after electroejaculation were higher for all electroejaculation treatments than for restraint alone. The increases in serum progesterone concentrations were significantly lower at 5 and 20 minutes after electroejaculation with epidural anesthesia than with no anesthesia. However, the change in cortisol or progesterone concentrations did not differ after electroejaculation when comparing the conventional probe or a segmented probe at any time during the study. Subjective scoring showed no differences among electroejaculation methods. Use of epidural anesthesia was beneficial in reducing progesterone, one indicator of an endocrine stress response to electroejaculation.
通过检测10头赫里福德公牛连续血样中皮质醇和孕酮的浓度变化，来量化其对不同电射精方法的应激反应。处理方式包括保定（对照），以及使用带有分段电极的直肠探头或传统非分段电极进行电射精，有无利多卡因尾段硬膜外麻醉。采用主观评分系统评估对不同电射精方法的行为反应。所有电射精处理后血清皮质醇和孕酮浓度的升高均高于单纯保定。硬膜外麻醉电射精后5分钟和20分钟时血清孕酮浓度的升高显著低于未麻醉时。然而，在研究期间的任何时间，比较传统探头或分段探头电射精后，皮质醇或孕酮浓度的变化并无差异。主观评分显示电射精方法之间无差异。硬膜外麻醉有助于降低孕酮，这是电射精内分泌应激反应的一个指标。
