Savaş Cağri, Ozgüner Meltem, Ozgüner Faruk, Delibaş Namik
Suleyman Demirel University Medical School, Department of Pediatric Surgery, Isparta, Turkey.
Int Urol Nephrol. 2003;35(2):237-45. doi: 10.1023/b:urol.0000020288.58686.5b.
BACKGROUND/PURPOSE: Unilateral testicular torsion can cause histologic damage, consisting of aspermatogenesis and tubular atrophy, in the contralateral testis human chorionic gonadotropin (HCG) treatment is widely used in undescended testis, and has been shown to improve histomorphometric alterations beside the testicular descent. However, the role of HCG in testicular torsion has not been investigated before. Therefore, this experimental study was conducted to evaluate the effects of HCG treatment on contralateral testicular histology and function in unilateral testicular torsion.
Forty adult male Wistar rats were randomized into 4 groups: SHAM, SHAM+HCG, TORSION, and TORSION+HCG. Torsion was created by twisting the righ testis 720 degrees and maintained by fixing it to the scrotum. HCG treatment started 24 hours after the torsion at a dose of 100 IU/kg, twice weekly for three weeks. Left orchiectomy was performed one month after the torsion and removed testes were immersed in Bouin's fixative for histopathological evaluation. Mean seminiferous tubule diameter (MSTD) was measured and Johnsen's score was calculated. Blood samples were taken for assaying serum testosteron level.
Unilateral testicular torsion resulted in a significant decrease in spermatogenesis and MSTD on the contralateral side. Serum testosteron level was also reduced. HCG treatment improved these parameters in the contralateral 'untwisted' testis beside the serum testosteron.
Our data demonstrates that unilateral testicular torsion adversely effects its counterpart. HCG treatment improves contralateral histomorphometric alterations and serum testosteron in unilateral torsion.
背景/目的：单侧睾丸扭转可导致对侧睾丸出现组织学损伤，包括无精子生成和曲细精管萎缩。人绒毛膜促性腺激素（HCG）治疗广泛应用于隐睾症，且已证明除了能促进睾丸下降外，还可改善组织形态学改变。然而，HCG在睾丸扭转中的作用此前尚未得到研究。因此，本实验研究旨在评估HCG治疗对单侧睾丸扭转对侧睾丸组织学及功能的影响。
40只成年雄性Wistar大鼠被随机分为4组：假手术组（SHAM）、假手术+HCG组（SHAM+HCG）、扭转组（TORSION）和扭转+HCG组（TORSION+HCG）。通过将右侧睾丸扭转720度并固定于阴囊来造成扭转。扭转后24小时开始进行HCG治疗，剂量为100 IU/kg，每周两次，共三周。扭转后一个月进行左侧睾丸切除术，切除的睾丸浸入Bouin固定液中进行组织病理学评估。测量平均曲细精管直径（MSTD）并计算Johnsen评分。采集血样检测血清睾酮水平。
单侧睾丸扭转导致对侧睾丸精子生成及MSTD显著降低。血清睾酮水平也降低。HCG治疗改善了对侧“未扭转”睾丸的这些参数以及血清睾酮水平。
我们的数据表明单侧睾丸扭转会对其对侧产生不利影响。HCG治疗可改善单侧扭转中对侧的组织形态学改变及血清睾酮水平。
