PerlPrimer：用于标准PCR、亚硫酸氢盐PCR和实时PCR的跨平台图形化引物设计工具。
PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR.
作者信息
Marshall Owen J
机构信息
Murdoch Childrens Research Institute, Royal Childrens Hospital, Flemington Road, Parkville, Vic., 3052, Australia.
出版信息
Bioinformatics. 2004 Oct 12;20(15):2471-2. doi: 10.1093/bioinformatics/bth254. Epub 2004 Apr 8.
UNLABELLED
PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.
AVAILABILITY
Open-source and freely available from http://perlprimer.sourceforge.net.
未标注
PerlPrimer是一款跨平台图形用户界面应用程序，用于设计标准PCR、亚硫酸氢盐PCR、实时PCR以及测序的引物。该程序集成了高度精确的解链温度和引物二聚体预测算法，并具备诸如从Ensembl检索序列以及对引物对进行BLAST搜索等强大工具。其目的是实现引物设计过程的自动化和简化。
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