Wang Jiren, Li Kuo-Bin, Sung Wing-Kin
BioInformatics Institute, 30 Biopolis Street, #07-01 Matrix, Singapore 138671.
Bioinformatics. 2004 Oct 12;20(15):2473-5. doi: 10.1093/bioinformatics/bth259. Epub 2004 Apr 8.
G-PRIMER, a web-based primer design program, has been developed to compute a minimal primer set specifically annealed to all the open reading frames in a given microbial genome. This program has been successfully used in the microarray experiment for analyzing the expression of genes in the Xanthomonas campestris genome.
It is available at http://mammoth.bii.a-star.edu.sg/gprimer/. Its source code is available upon request.
G-PRIMER是一个基于网络的引物设计程序,旨在计算出一套最小化引物集,该引物集能特异性地退火到给定微生物基因组中的所有开放阅读框。该程序已成功应用于微阵列实验,用于分析野油菜黄单胞菌基因组中基因的表达。