Ohkia Akira, Hu Youji, Wang Min, Garcia Fernando U, Stearns Mark E
Drexel University College of Medicine, Department of Pathology and Laboratory Medicine, Philadelphia, Pennsylvania 19102, USA.
Clin Cancer Res. 2004 Apr 1;10(7):2452-8. doi: 10.1158/1078-0432.ccr-03-0170.
The purpose of this study was to characterize a novel gene/protein associated with prostate cancer, termed prostate cancer-associated diagnostic marker-1 [PCADM-1 (Hu Y, Wang M, Garcia FU, Aoyaki K, Stearns ME. Identification of PCADM-1 as a novel diagnostic marker for prostate cancer, submitted for publication)].
Immunological studies revealed that rabbit polyclonal antibodies generated against recombinant PCADM-1 specifically recognize the protein in crude protein extracts from a variety of prostate cancer cell lines (i.e., PC-3 ML, LNCaP, DU145, and CPTX-1532) and prostate cancer tissue. Combined immunolabeling and in situ hybridization studies demonstrated that PCADM-1 mRNA was expressed by the luminal epithelial cells of prostate cancer glands and was not expressed by high-grade prostatic intraepithelial neoplasia or HPV-MLC7 cells. Immunolabeling studies of tissue arrays from biopsies of archival material (n = 200 samples) confirmed that PCADM-1 was expressed by the luminal epithelial cells of prostate cancer.
Taken together, the data suggest that PCADM-1 is a specific marker for human prostate cancer.
本研究旨在鉴定一种与前列腺癌相关的新基因/蛋白，命名为前列腺癌相关诊断标志物-1[PCADM-1（胡Y，王M，加西亚FU，青柳K，斯特恩斯ME。PCADM-1作为前列腺癌新诊断标志物的鉴定，已提交发表）]。
免疫学研究表明，针对重组PCADM-1产生的兔多克隆抗体能够特异性识别来自多种前列腺癌细胞系（即PC-3 ML、LNCaP、DU145和CPTX-1532）以及前列腺癌组织的粗蛋白提取物中的该蛋白。免疫标记与原位杂交联合研究显示，PCADM-1 mRNA在前列腺癌腺管的腔上皮细胞中表达，而在高级别前列腺上皮内瘤变或HPV-MLC7细胞中不表达。对存档材料活检组织芯片（n = 200个样本）的免疫标记研究证实，PCADM-1在前列腺癌的腔上皮细胞中表达。
综上所述，数据表明PCADM-1是人类前列腺癌的特异性标志物。
