Ezra David, Castillo Uvidelio F, Strobel Gary A, Hess Wilford M, Porter Heidi, Jensen James B, Condron Margaret A M, Teplow David B, Sears Joseph, Maranta Michelle, Hunter Michelle, Weber Barbara, Yaver Debbie
Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA.
Department of Integrated Biology, Brigham Young University, Provo, UT 84602, USA.
Microbiology (Reading). 2004 Apr;150(Pt 4):785-793. doi: 10.1099/mic.0.26645-0.
Coronamycin is a complex of novel peptide antibiotics with activity against pythiaceous fungi and the human fungal pathogen Cryptococcus neoformans. It is also active against the malarial parasite, Plasmodium falciparum, with an IC(50) of 9.0 ng ml(-1). Coronamycin is produced by a verticillate Streptomyces sp. isolated as an endophyte from an epiphytic vine, Monstera sp., found in the Manu region of the upper Amazon of Peru. Bioassay-guided fractionation of the fermentation broths of this endophyte on silica gel and HPLC chromatography yielded two principal, inseparable, peptides with masses of 1217.9 and 1203.8 Da. Three other minor, but related components, are also present in the preparation. Amino acid analysis of coronamycin revealed residues of component 1, component 2, methionine, tyrosine and leucine at a ratio of 2:2:1:1:3. Other compounds with antifungal activities are also produced by this endophytic streptomycete.
冠霉素是一种新型肽类抗生素复合物，对腐霉科真菌和人类真菌病原体新型隐球菌具有活性。它对疟原虫恶性疟原虫也有活性，半数抑制浓度（IC₅₀）为9.0纳克/毫升。冠霉素由一株轮生链霉菌产生，该菌株是从秘鲁亚马逊上游马努地区一种附生藤本植物龟背竹中分离出的内生菌。对该内生菌发酵液进行硅胶生物测定导向分级分离和高效液相色谱分离，得到两个主要的、无法分离的肽段，分子量分别为1217.9和1203.8道尔顿。制剂中还存在其他三个次要但相关的成分。对冠霉素的氨基酸分析显示，成分1、成分2、蛋氨酸、酪氨酸和亮氨酸的残基比例为2:2:1:1:3。这种内生链霉菌还产生其他具有抗真菌活性的化合物。
