对来自不同来源的10种皮炎芽生菌酵母相裂解物抗原进行的比较血清学评估。

Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sources.

作者信息

Abuodeh R O, Chester E M, Scalarone G M

机构信息

College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.

出版信息

Mycoses. 2004 Apr;47(3-4):143-9. doi: 10.1111/j.1439-0507.2004.00972.x.

DOI:10.1111/j.1439-0507.2004.00972.x

PMID:15078431

Abstract

Yeast phase lysate antigens from 10 isolates of Blastomyces dermatitidis (dog, ERC-2 and T-58; T-27, polar bear; woodpile, ER-3; bat lung, 48938; human, B5929, B5895, B5896, B5931, and CAPP) from different geographical regions, in addition to a Histoplasma capsulatum (G217B) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme-linked immunosorbent assay. In addition, the lysate antigens were also assayed against histoplasmosis-positive human serum samples to study their cross-reactivity. Variable results were obtained with T-58 and T-27 exhibiting the greatest reactivity. We also noticed that the lysate did not react consistently to serum samples across species with lesser reactivity evidenced when testing dog sera. Finally, T-58 gave the highest cross-reactivity with histoplasmosis-positive sera. The study may prove valuable in the development of antigen candidates for blastomycosis serodiagnosis.

摘要

来自不同地理区域的10株皮炎芽生菌（犬，ERC - 2和T - 58；北极熊，T - 27；木堆，ER - 3；蝙蝠肺，48938；人，B5929、B5895、B5896、B5931和CAPP）的酵母相裂解物抗原，除荚膜组织胞浆菌（G217B）裂解物制剂外，使用间接酶联免疫吸附测定法，针对犬、兔和患有芽生菌病的人类血清标本的反应性进行了比较。此外，还针对组织胞浆菌病阳性的人类血清样本检测了裂解物抗原，以研究它们的交叉反应性。T - 58和T - 27表现出最大的反应性，得到了不同的结果。我们还注意到，裂解物对不同物种的血清样本反应不一致，检测犬血清时反应性较低。最后，T - 58与组织胞浆菌病阳性血清的交叉反应性最高。该研究可能对芽生菌病血清诊断抗原候选物的开发具有重要价值。